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Artículo

Short transmembrane domains with high-volume exoplasmic halves determine retention of Type II membrane proteins in the Golgi complex

Quiroga, RodrigoIcon ; Trenchi, AlejandraIcon ; Gonzalez Montoro, Ayelén; Valdez, Javier EstebanIcon ; Maccioni, Hugo Jose FernandoIcon
Fecha de publicación: 12/2013
Editorial: Company Of Biologists
Revista: Journal Of Cell Science
ISSN: 0021-9533
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

It is still unclear why some proteins that travel along the secretory pathway are retained in the Golgi complex whereas others make their way to the plasma membrane. Recent bioinformatic analyses on a large number of single-spanning membrane proteins support the hypothesis that specific features of the transmembrane domain (TMD) are relevant to the sorting of these proteins to particular organelles. Here we experimentally test this hypothesis for Golgi and plasma membrane proteins. Using the Golgi SNARE protein Sft1 and the plasma membrane SNARE protein Sso1 from Saccharomyces cerevisiae as model proteins, we modified the length of their TMDs and the volume of their exoplasmic hemi-TMD, and determined their subcellular localization both in yeast and mammalian cells. We found that short TMDs with high-volume exoplasmic hemi-TMDs confer Golgi membrane residence, whereas TMDs with low-volume exoplasmic hemi-TMDs, either short or long, confer plasma membrane residence to these proteins. Results indicate that the shape of the exoplasmic hemi-TMD, in addition to the length of the entire TMD, determine retention in the Golgi or exit to the plasma membrane of Type II membrane proteins.
Palabras clave: Transmembrane Domain , Golgi , Traffic , Yeast
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/10971
DOI: http://dx.doi.org/10.1242/jcs.130658
URL: http://jcs.biologists.org/content/126/23/5344
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Articulos(IMBIV)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Quiroga, Rodrigo; Trenchi, Alejandra; Gonzalez Montoro, Ayelén; Valdez, Javier Esteban; Maccioni, Hugo Jose Fernando; Short transmembrane domains with high-volume exoplasmic halves determine retention of Type II membrane proteins in the Golgi complex; Company Of Biologists; Journal Of Cell Science; 126; 12-2013; 5344-5349
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