Mostrar el registro sencillo del ítem
dc.contributor.author
Lorenzo Lopez, Juan Ramiro
dc.contributor.author
Onizuka, Michiho
dc.contributor.author
Defrance, Matthieu
dc.contributor.author
Laurent, Patrick
dc.date.available
2020-07-20T14:53:23Z
dc.date.issued
2020-06
dc.identifier.citation
Lorenzo Lopez, Juan Ramiro; Onizuka, Michiho; Defrance, Matthieu; Laurent, Patrick; Combining single-cell RNA-sequencing with a molecular atlas unveils new markers for Caenorhabditis elegans neuron classes; Oxford University Press; Nucleic Acids Research; 48; 13; 6-2020; 1-16
dc.identifier.issn
0305-1048
dc.identifier.uri
http://hdl.handle.net/11336/109644
dc.description.abstract
Single-cell RNA-sequencing (scRNA-seq) of the Caenorhabditis elegans nervous system offers the unique opportunity to obtain a partial expression profile for each neuron within a known connectome. Building on recent scRNA-seq data and on a molecular atlas describing the expression pattern of ∼800 genes at the single cell resolution, we designed an iterative clustering analysis aiming to match each cell-cluster to the ∼100 anatomically defined neuron classes of C. elegans. This heuristic approach successfully assigned 97 of the 118 neuron classes to a cluster. Sixty two clusters were assigned to a single neuron class and 15 clusters grouped neuron classes sharing close molecular signatures. Pseudotime analysis revealed a maturation process occurring in some neurons (e.g. PDA) during the L2 stage. Based on the molecular profiles of all identified neurons, we predicted cell fate regulators and experimentally validated unc-86 for the normal differentiation of RMG neurons. Furthermore, we observed that different classes of genes functionally diversify sensory neurons, interneurons and motorneurons. Finally, we designed 15 new neuron class-specific promoters validated in vivo. Amongst them, 10 represent the only specific promoter reported to this day, expanding the list of neurons amenable to genetic manipulations.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
SCRNA-SEQ
dc.subject
CAENORHABDITIS ELEGANS
dc.subject
NEURONS
dc.subject
GENE EXPRESSION PROFILES
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Combining single-cell RNA-sequencing with a molecular atlas unveils new markers for Caenorhabditis elegans neuron classes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-01T20:33:56Z
dc.identifier.eissn
1362-4962
dc.journal.volume
48
dc.journal.number
13
dc.journal.pagination
1-16
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Lorenzo Lopez, Juan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Université Libre de Bruxelles; Bélgica
dc.description.fil
Fil: Onizuka, Michiho. Université Libre de Bruxelles; Bélgica
dc.description.fil
Fil: Defrance, Matthieu. Université Libre de Bruxelles; Bélgica
dc.description.fil
Fil: Laurent, Patrick. Université Libre de Bruxelles; Bélgica
dc.journal.title
Nucleic Acids Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa486/5857708
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/nar/gkaa486
Archivos asociados