Artículo
Cellulolytic ability of a novel promising Irpex lacteus (Basidiomycota: Polyporales) strain from the subtropical rainforest of Misiones province, Argentina
Giorgio, Ernesto Martín
; Villalba, Laura; Robledo, Gerardo Lucio
; Zapata, Pedro Dario
; Saparrat, Mario Carlos Nazareno
Fecha de publicación:
08/2018
Editorial:
Revista de Biología Tropical
Revista:
Revista de Biología Tropical
ISSN:
0034-7744
e-ISSN:
0034-7744
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The cellulolytic activity of fungi growing in the subtropical rainforest of Misiones (Argentina) represents a challenge in the technological development of the production of cellulosic bioethanol in the region using native sources. These fungi are promising in the obtaining of sustainable enzyme cocktails using their enzymes. Cellulolytic ability of 22 white-rot fungi isolated from the subtropical rainforest of Misiones-Argentina in agar medium with two types of cellulosic substrates, carboxy-methylcellulose or crystalline cellulose, were comparatively analyzed, and the activity of two cellulolytic enzymes was evaluated in liquid medium. Although all isolates were able to grow and degrade both substrates in agar medium, and to produce total cellulase Filter paper (FPase) and endo-β-1,4-glucanase (EG) activities in broth, the isolate Irpex sp. LBM 034 showed the greatest enzymatic levels (FPase, 65.45 U L-1; EG, 221.21 U L-1). Therefore, the ITS sequence of this fungus was sequenced and analyzed through a phylogenetic analysis. These results indicate that the isolate LBM 034, corresponding to Irpex lacteus, has a promising cellulolytic ability and enzymes such as EG useful in sustainable saccharification of cellulosic materials in the region
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Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Citación
Giorgio, Ernesto Martín; Villalba, Laura; Robledo, Gerardo Lucio; Zapata, Pedro Dario; Saparrat, Mario Carlos Nazareno; Cellulolytic ability of a novel promising Irpex lacteus (Basidiomycota: Polyporales) strain from the subtropical rainforest of Misiones province, Argentina; Revista de Biología Tropical; Revista de Biología Tropical; 66; 8-2018; 1034-1045
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