Artículo
Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying
Martín Giménez, Virna Margarita
; Russo, Marcos Guillermo
; Narda, Griselda Edith
; Fuentes, Lucía B.; Mazzei, Luciana Jorgelina
; Gamarra Luques, Carlos Diego
; Kassuha, Diego Enrique
; Manucha, Walter Ariel Fernando
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Fecha de publicación:
11/2019
Editorial:
Institution of Engineering and Technology
Revista:
Iet Nanobiotechnology
ISSN:
1751-8741
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Drug encapsulation in nanocarriers such as polymeric nanoparticles (Nps) may help to overcome the limitations associated with cannabinoids. In this sense, our work aimed to highlight the use of electrospraying technique for the development of carrier Nps of anandamide (AEA), an endocannabinoid with attractive pharmacological effects but underestimated due to its unfavorable physicochemical and pharmacokinetic properties added to its undesirable effects at the level of the central nervous system. We characterized physicochemically and evaluated in vitro biological activity of anandamide/ε-polycaprolactone nanoparticles (Nps-AEA/PCL) obtained by electrospraying in epithelial cells of human proximal tubule (HK2), to prove the utility of this method and to validate the biological effect of Nps-AEA/PCL. We obtained particles from 100 to 900 nm of diameter with a predominance of 200 a 400 nm. Their zeta potential was -20±1.86 mV. We demonstrated the stable encapsulation of AEA in Nps-AEA/PCL, as well as its dose-dependent capacity to induce the expression of iNOS and NO levels and to decrease the Na+/K+ ATPase activity in HK2 cells. Obtaining Nps-AEA/PCL by electrospraying would represent a promising methodology for a novel AEA pharmaceutical formulation development with optimal physicochemical properties, physical stability and biological activity on HK2 cells.
Palabras clave:
NANOBIOTECHNOLOGY
,
NANO-DRUG
,
ANANDAMIDE
,
HYPERTENSION
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - SAN JUAN)
Articulos de CENTRO CIENTIFICO TECNOLOGICO CONICET - SAN JUAN
Articulos de CENTRO CIENTIFICO TECNOLOGICO CONICET - SAN JUAN
Articulos(IMBECU)
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Articulos(INTEQUI)
Articulos de INST. DE INVEST. EN TECNOLOGIA QUIMICA
Articulos de INST. DE INVEST. EN TECNOLOGIA QUIMICA
Citación
Martín Giménez, Virna Margarita; Russo, Marcos Guillermo; Narda, Griselda Edith; Fuentes, Lucía B.; Mazzei, Luciana Jorgelina; et al.; Synthesis, physicochemical characterization and biological activity of anandamide/ε-polycaprolactone nanoparticles obtained by electrospraying; Institution of Engineering and Technology; Iet Nanobiotechnology; 14; 1; 11-2019; 86-93
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