Mostrar el registro sencillo del ítem Chui, Kitty Trivedi, Alpa Cheng, C. Yan Cherbavaz, Diana B. Dazin, Paul F. Huynh, Ai Lam Thu Mitchel, James B. Rabinovich, Gabriel Adrian Noble Haeusslein, Linda J. John, Constance M. 2017-01-04T20:00:33Z 2011-05
dc.identifier.citation Chui, Kitty; Trivedi, Alpa; Cheng, C. Yan; Cherbavaz, Diana B. ; Dazin, Paul F.; et al.; Characterization and functionality of proliferative human sertoli cells; Cognizant Communication Corp; Cell Transplantation; 20; 5; 5-2011; 619-635
dc.identifier.issn 0963-6897
dc.description.abstract It has long been thought that mammalian Sertoli cells are terminally differentiated and nondividing postpuberty. For most previous in vitro studies immature rodent testes have been the source of Sertoli cells and these have shown little proliferative ability when cultured. We have isolated and characterized Sertoli cells from human cadaveric testes from seven donors ranging from 12 to 36 years of age. The cells proliferated readily in vitro under the optimized conditions used with a doubling time of approximately 4 days. Nuclear 5-ethynyl-2´-deoxyuridine (EdU) incorporation confirmed that dividing cells represented the majority of the population. Classical Sertoli cell ultrastructural features, lipid droplet accumulation, and immunoexpression of GATA-4, Sox9, and the FSH receptor (FSHr) were observed by electron and fluorescence microscopy, respectively. Flow cytometry revealed the expression of GATA-4 and Sox9 by more than 99% of the cells, and abundant expression of a number of markers indicative of multipotent mesenchymal cells. Low detection of endogenous alkaline phosphatase activity after passaging showed that few peritubular myoid cells were present. GATA-4 and SOX9 expression were confirmed by reverse transcription polymerase chain reaction (RT-PCR), along with expression of stem cell factor (SCF), glial cell line-derived neurotrophic factor (GDNF), and bone morphogenic protein 4 (BMP4). Tight junctions were formed by Sertoli cells plated on transwell inserts coated with fibronectin as revealed by increased transepithelial electrical resistance (TER) and polarized secretion of the immunoregulatory protein, galectin-1. These primary Sertoli cell populations could be expanded dramatically in vitro and could be cryopreserved. The results show that functional human Sertoli cells can be propagated in vitro from testicular cells isolated from adult testis. The proliferative human Sertoli cells should have important applications in studying infertility, reproductive toxicology, testicular cancer, and spermatogenesis, and due to their unique biological properties potentially could be useful in cell therapy.
dc.format application/pdf
dc.language.iso eng
dc.publisher Cognizant Communication Corp
dc.rights info:eu-repo/semantics/openAccess
dc.subject SERTOLI CELLS
dc.subject CELL CYCLE
dc.subject GALECTINS
dc.subject.classification Fisiología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Characterization and functionality of proliferative human sertoli cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion 2016-12-30T13:43:26Z
dc.identifier.eissn 1555-3892
dc.journal.volume 20
dc.journal.number 5
dc.journal.pagination 619-635
dc.journal.pais Estados Unidos
dc.journal.ciudad Nueva York
dc.description.fil Fil: Chui, Kitty. MandalMed Inc. ; Estados Unidos
dc.description.fil Fil: Trivedi, Alpa. MandalMed Inc.; Estados Unidos. University of California; Estados Unidos
dc.description.fil Fil: Cheng, C. Yan. Lonza Walkersville; Estados Unidos
dc.description.fil Fil: Cherbavaz, Diana B. . MandalMed Inc.; Estados Unidos
dc.description.fil Fil: Dazin, Paul F.. MandalMed; Estados Unidos
dc.description.fil Fil: Huynh, Ai Lam Thu. MandalMed Inc.; Estados Unidos
dc.description.fil Fil: Mitchel, James B.. Lonza Walkersville; Estados Unidos
dc.description.fil Fil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil Fil: Noble Haeusslein, Linda J.. University of California; Estados Unidos
dc.description.fil Fil: John, Constance M.. MandalMed Inc.; Estados Unidos
dc.journal.title Cell Transplantation
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/

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