Artículo
New Sequence Variations in Spermatogenesis Candidates Genes
Poli, María Noelia
; Fernandez Iriarte, Pedro Jose
; Iudica, Celia Estela; Zanier, Justo Hector Mario; Coco, Roberto
Fecha de publicación:
09/2015
Editorial:
Brazilian Society of Assisted Reproduction
Revista:
JBRA Assisted Reproduction
ISSN:
1518 0557
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this paper was to estimate the frequency and types of mutations in key candidate genes involved in spermatogenesis, and its potential value as a cause of azoospermia / cryptozoospermia.Patients and Methods: the sequencing of the coding region of the genes DBY, RBMY, DAZ, CDY and BPY2, excluding the promoter region was performed in a series of 25 patients with azoospeermia or severe oligozoospermia without AZF microdeletions. Exon 3 of DAZL gene (DAL3) was also sequenced. The sequences obtained were analyzed by ProSeq, DnaSP v5 and compared with the database through Blastn and tblastx.Results and conclusions: 16 of the 25 patients showed some type of variants, such as transversions, transitions, deletions and / or insertions in the DAZ, DAZL, CDY and RBMY genes. The mutated sequences presented between 97 and 99% homology with the specific protein of every gene, except in DAZL (73%) and DAZ (94%) proteins. The variants found have not been described previously, suggesting they could be mutations that might affect protein function.
Palabras clave:
Male infertility
,
spermatogenesis candidate genes
,
New sequence variants
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Colecciones
Articulos(IIMYC)
Articulos de INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Articulos de INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Citación
Poli, María Noelia; Fernandez Iriarte, Pedro Jose; Iudica, Celia Estela; Zanier, Justo Hector Mario; Coco, Roberto; New Sequence Variations in Spermatogenesis Candidates Genes; Brazilian Society of Assisted Reproduction; JBRA Assisted Reproduction; 19; 4; 9-2015; 216-222
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