Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes

Abstract

Aims: Cells limit the cell number of dense biofilms by releasing self-inhibitory molecules. Here, we aim to assess the effectiveness of yeast quorum sensing (QS) molecules and the antifungal agent natamycin against yeast biofilms of strains commonly isolated from fruit juice ultrafiltration membranes.Methods and results.Yeast QS molecules such as tyrosol, 2-phenylethanol and farnesol were detected by solvent extraction and HS SPME GC-MS in C. tropicalis cultures. The effect of quorum sensing (QS) molecules on mono and multi species biofilms formed by Rhodotorula mucilaginosa, Candida tropicalis, Candida krusei and Candida kefyr was evaluated by plate count and epifluorescence microscopy.Farnesol caused a decrease in cell number and disrupted mono and multispecies yeast biofilms during adhesion (0.6 mmol l-1). 2-phenyl ethanol 1.2 mmol l-1 stimulated biofilm density and increased cell number in both mono and multispecies biofilms, while tyrosol did not show effects when tested against C. tropicalis biofilms (0.05-1.2 mmol l-1). Natamycin caused a strong decrease on cell number and disruption of biofilm structure in C. tropicalis biofilms at high concentrations (0.3 to 1.2 mmol l-1). The combination of farnesol 0.6 mmol l-1 and natamycin at 0.01 mmol l-1, the maximum concentration of natamycin accepted for direct addition into fruit juices, effectively reduced cell counts and disrupted the structure of C. tropicalis biofilms.ConclusionFarnesol 0.6 mmol l-1 significantly increased the inhibition exerted by natamycin 0.01 mmol l-1 (¡­5 ppm) reducing biofilm development from juice on stainless steel surfaces. Significance and impact of the studyThese results support the use of QS molecules as biofilm inhibitors in beverages and would certainly inspire the design of novel preservative and cleaning products for the food industry based on combinatory approaches.