Artículo
A novel fluorimetric method for glyphosate and AMPA determination with NBD-Cl and MCR-ALS
Pérez, Ana Laura
; Tibaldo, Giuliana; Sánchez, Germán Hugo
; Siano, Gabriel German
; Marsili, Nilda Raquel; Schenone, Agustina Violeta
Fecha de publicación:
05/2019
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
ISSN:
1386-1425
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We report the development of a new analytical method for the quantification of N-(phosphonomethyl)glycine (glyphosate) and (aminomethyl)phosphonic acid (AMPA) by combining spectrofluorimetry and multivariate calibration. In this study, fluorescence spectroscopy was used to quantify glyphosate and AMPA, which were previously derivatized with the fluorogenic reagent: 4-chloro-7-nitrobenzofurazan (NBD-Cl). Fluorescence excitation-emission matrices (EEM) were recorded by exciting between 400 and 500 nm, and measuring the emission between 500 and 610 nm. The second-order data obtained were processed using the Multivariate Curve Resolution with Alternating Least Square (MCR-ALS) methodology. The developed method was used to predict different concentrations of glyphosate and AMPA in validation samples. In addition, the presence of the herbicide was evaluated in real samples: a commercial formulation and a water sample from a cultivated area. For this purpose, the standard addition method was used to study the matrix effect in each case. The ranges of working concentrations obtained for this new method are in agreement with the amounts found in surface water samples near a direct sowing soybean growing region in Argentina.
Palabras clave:
GLYPHOSATE
,
AMPA
,
NBD-CL
,
SPECTROFLUORIMETRY
,
MCR-ALS
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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Articulos(SINC(I))
Articulos de INST. DE INVESTIGACION EN SEÑALES, SISTEMAS E INTELIGENCIA COMPUTACIONAL
Articulos de INST. DE INVESTIGACION EN SEÑALES, SISTEMAS E INTELIGENCIA COMPUTACIONAL
Citación
Pérez, Ana Laura; Tibaldo, Giuliana; Sánchez, Germán Hugo; Siano, Gabriel German; Marsili, Nilda Raquel; et al.; A novel fluorimetric method for glyphosate and AMPA determination with NBD-Cl and MCR-ALS; Pergamon-Elsevier Science Ltd; Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy; 214; 5-2019; 119-128
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