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dc.contributor.author
Grippo, Lucía Daniela
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Rudi, Juan Manuel
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de Zan, María Mercedes
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Giorello, Antonella
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Antuña, Sebastián
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Prieto, Claudio
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Veaute, Carolina Melania Isabel
dc.contributor.author
Müller, Diana María
dc.date.available
2020-06-23T12:49:37Z
dc.date.issued
2019-05
dc.identifier.citation
Grippo, Lucía Daniela; Rudi, Juan Manuel; de Zan, María Mercedes; Giorello, Antonella; Antuña, Sebastián; et al.; Activity-Structure Study on the Peptide Fraction of AG2: a Potent In Vitro Transfection Agent; Humana Press; Applied Biochemistry And Biotechnology; 5-2019
dc.identifier.issn
0273-2289
dc.identifier.uri
http://hdl.handle.net/11336/107891
dc.description.abstract
Gemini-based amphiphiles are candidates for biomedical applications. In fact, most of thegemini compounds described in the literature have been prepared to be used as new syntheticvectors in gene transfection. Our group carried out an activity-structure study starting from thestructure of the gemini [AG2-C18/]2, which is an effective in vitro transfection reagent. Wesynthesized a series of novel amphiphilic amino acid derivatives of low molecular weight,named AGn-Cm(N), in which the same apolar region (m) of oleic or palmitic acid wasmaintained and the peptide region was modified by amino acid insertions, deletions, andsubstitutions. We also determined the transfection efficiency, critical micelle concentration,particle size, and ζ-potential for these derivatives. Amphiphiles AG10-C16and AG 10-C18weremore active at a lower N/P ratio than AG2-C18. These amphiphiles showed no activity whenlysine was replaced by ornithine, and the activity of all derivatives increased when there weremore ornithine residues and a W/O = 1 ratio in the peptide region. It can be said that for AG10-C16, these two structural requirements on the amino acid portion predominated over the type ofaliphatic chain used.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AMPHIPHILE
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N-ACYLATED
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CYSTEINE
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GEMINI
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ORNITHINE
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TRANSFECTION
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Activity-Structure Study on the Peptide Fraction of AG2: a Potent In Vitro Transfection Agent
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-06-16T13:41:33Z
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Oregon
dc.description.fil
Fil: Grippo, Lucía Daniela. Laboratorio de Química Aplicada; Argentina
dc.description.fil
Fil: Rudi, Juan Manuel. Laboratorio de Química Aplicada; Argentina
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Fil: de Zan, María Mercedes. Laboratorio de Control de Medicamentos; Argentina
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Fil: Giorello, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Antuña, Sebastián. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
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Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
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Fil: Veaute, Carolina Melania Isabel. Laboratorio de Inmunología Básica; Argentina
dc.description.fil
Fil: Müller, Diana María. Laboratorio de Química Aplicada; Argentina
dc.journal.title
Applied Biochemistry And Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12010-019-02999-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12010-019-02999-y
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