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dc.contributor.author
Rêgo, Juciane Vaz
dc.contributor.author
Duarte, Ana Paula
dc.contributor.author
Liarte, Daniel Barbosa
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de Carvalho Sousa, Francirlene
dc.contributor.author
Barreto, Humberto Medeiros
dc.contributor.author
Bua, Jacqueline Elena
dc.contributor.author
Romanha, Alvaro José
dc.contributor.author
Rádis Baptista, Gandhi
dc.contributor.author
Murta, Silvane Maria Fonseca
dc.date.available
2020-06-18T15:48:17Z
dc.date.issued
2015-01
dc.identifier.citation
Rêgo, Juciane Vaz; Duarte, Ana Paula; Liarte, Daniel Barbosa; de Carvalho Sousa, Francirlene; Barreto, Humberto Medeiros; et al.; Molecular characterization of Cyclophilin (TcCyP19) in Trypanosoma cruzi populations susceptible and resistant to benznidazole; Academic Press Inc Elsevier Science; Experimental Parasitology; 148; 1-2015; 73-80
dc.identifier.issn
0014-4894
dc.identifier.uri
http://hdl.handle.net/11336/107611
dc.description.abstract
Cyclophilin (TcCyP19), a peptidyl-prolyl cis/trans isomerase, is a key molecule with diverse biological functions that include roles in molecular chaperoning, stress response, immune modulation, and signal transduction. In this respect, TcCyP19 could serve as a potential drug target in diseasecausing parasites. Previous studies employing proteomics techniques have shown that the TcCyP19 isoform was more abundant in a benznidazole (BZ)-resistant Trypanosoma cruzi population than in its susceptible counterpart. In this study, TcCyP19 has been characterized in BZ-susceptible and BZresistant T. cruzi populations. Phylogenetic analysis revealed a clear dichotomy between Cyphophilin A (CyPA) sequences from trypanosomatids and mammals. Sequencing analysis revealed that the amino acid sequences of TcCyP19 were identical among the T. cruzi samples analyzed. Southern blot analysis showed that TcCyP19 is a single-copy gene, located in chromosomal bands varying in size from 0.68 to 2.2 Mb, depending on the strain of T. cruzi. Northern blot and qPCR indicated that the levels of TcCyP19 mRNA were two-fold higher in drug-resistant T. cruzi populations than in their drugsusceptible counterparts. Similarly, as determined by two-dimensional gel electrophoresis immunoblot, the expression of TcCyP19 protein was increased to the same degree in BZ-resistant T. cruzi populations. No differences in TcCyP19 mRNA and protein expression levels were observed between the susceptible and the naturally resistant T. cruzi strains analyzed. Taken together, these data indicate that cyclophilin TcCyP19 expression is up-regulated at both transcriptional and translational levels in T. cruzi populations that were in vitro-induced and in vivo-selected for resistance to BZ.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Trypanosoma cruzi
dc.subject
Ciclofilinas
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resistencia benznidazol
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sobreexpresion de TcCyP19
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Tecnologías que involucran la identificación de ADN, proteínas y enzimas, y cómo influyen en el conjunto de enfermedades y mantenimiento del bienestar
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Biotecnología de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Molecular characterization of Cyclophilin (TcCyP19) in Trypanosoma cruzi populations susceptible and resistant to benznidazole
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-06-18T15:10:41Z
dc.journal.volume
148
dc.journal.pagination
73-80
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Rêgo, Juciane Vaz. Universidade Federal Do Piaui.; Brasil
dc.description.fil
Fil: Duarte, Ana Paula. Fundación Oswaldo Cruz; Brasil
dc.description.fil
Fil: Liarte, Daniel Barbosa. Universidade Federal Do Piaui.; Brasil
dc.description.fil
Fil: de Carvalho Sousa, Francirlene. Universidade Federal Do Piaui.; Brasil
dc.description.fil
Fil: Barreto, Humberto Medeiros. Universidade Federal Do Piaui.; Brasil
dc.description.fil
Fil: Bua, Jacqueline Elena. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Romanha, Alvaro José. Fundación Oswaldo Cruz; Brasil
dc.description.fil
Fil: Rádis Baptista, Gandhi. Universidade Federal Do Piaui.; Brasil
dc.description.fil
Fil: Murta, Silvane Maria Fonseca. Universidade Federal Do Piaui.; Brasil
dc.journal.title
Experimental Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/www.sciencedirect.com/science/article/abs/pii/S0014489414002689
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.exppara.2014.11.007
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