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dc.contributor.author
Toledano, Rosa
dc.contributor.author
Cortés, José
dc.contributor.author
Andini, Juan Carlos
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dc.contributor.author
Vázquez, Ana
dc.contributor.author
Villén, Jesús
dc.date.available
2017-01-03T14:41:59Z
dc.date.issued
2012-07
dc.identifier.citation
Toledano, Rosa; Cortés, José; Andini, Juan Carlos; Vázquez, Ana; Villén, Jesús; On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils; Elsevier Science; Journal Of Chromatography - A; 1256; 7-2012; 191-196
dc.identifier.issn
0021-9673
dc.identifier.uri
http://hdl.handle.net/11336/10731
dc.description.abstract
In the present work on-line derivatization is combined with on-line normal phase liquid chromatography-gas chromatography (NPLC-GC) to analyze total sterols in edible oils. The method uses the TOTAD interface with an additional LC injection valve to automatically introduce the derivatization reagent. The derivatization reaction takes place in the adsorbent material located inside the liner of the TOTAD interface. The samples were saponified with potassium hydroxide in an ethanolic solution and the unsaponifiable fraction was extracted with diethyl ether. The extract was then analyzed by online derivatization with on-line NPLC?GC, avoiding the laborious thin layer chromatography and off-line derivatization steps used in the Official European Union (EU) method. The relative standard deviations (RSDs) from the absolute peak area were lower than 14% except for campestanol. No variability in retention time was observed. Limits of detection (LODs) were less than 8.82 mg/kg. Different edible oils were analyzed using the proposed method and the results obtained were compared with those obtained using the Official EU method. There was good agreement between both methods for all the sterols analyzed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
On-Line Derivatization
dc.subject
On-Line Nplcgc
dc.subject
Totad Interface
dc.subject
Total Sterols
dc.subject.classification
Química Analítica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-01-02T18:28:05Z
dc.journal.volume
1256
dc.journal.pagination
191-196
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Toledano, Rosa. Universidad de Castilla-La Mancha; España
dc.description.fil
Fil: Cortés, José. Universidad de Castilla-La Mancha; España
dc.description.fil
Fil: Andini, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
dc.description.fil
Fil: Vázquez, Ana. Universidad de Castilla-La Mancha; España
dc.description.fil
Fil: Villén, Jesús. Universidad de Castilla-La Mancha; España
dc.journal.title
Journal Of Chromatography - A
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.chroma.2012.07.057
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021967312011120
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