Capítulo de Libro
Beta actin: its implication in the seminiferous tubule
Título del libro: Testis: Anatomy, Physiology and Pathology
Losinno, Antonella Denise
; Lopez, Luis Alberto
; Capani, Francisco
; Foscolo, Mabel Rosa
; Ibañez, Jorge Ernesto
; Cavicchia, Juan Carlos
Fecha de publicación:
2012
Editorial:
Nova Science Publishers, Inc.
ISBN:
978-1-61942-744-0
Idioma:
Inglés
Clasificación temática:
Resumen
Junctional devices in Sertoli cells play a key role in maturation and differentiation of germ cells. However, the cellular and subcellular organization of these specializations are still not totally understood. By combining light and electron microscopic techniques using â-actin immunolabeling and prosaposin and glutaredoxin antibodies to label Sertoli cytoplasm, we observed the structural (by confocal microscopy) and fine structural (by electron microscopy) organization of tight and adherent junctions, which are the morphological substrate of the blood testis barrier (BTB). The association to the beta actin also characterizes ectoplasmic specializations (ES) found at two different level of the seminiferous epithelium. We also used freeze fracture to analyze the characteristics of tubular bulbar complexes (TBC), a known component of apical ectoplasmic specializations. These different approaches also allowed us to study the complex arrangement of the actin cytoskeleton of Sertoli cells branches, which surround germ cells in different stages of the spermatogenic cycle. Our results show a consistent labelling for â-actin before, during and after the release of spermatozoa in the tubular lumen (spermiation) suggesting a significant role of the actin network in spermatic cell differentiation. In conclusion, significant interrelations among the â-actin network, the junctional complexes of the BTB and the ectoplasmic specializations were detected at different stages of the seminiferous cycle. â-actin immunolabeling and prosaposin and glutaredoxin antibodies to label Sertoli cytoplasm, we observed the structural (by confocal microscopy) and fine structural (by electron microscopy) organization of tight and adherent junctions, which are the morphological substrate of the blood testis barrier (BTB). The association to the beta actin also characterizes ectoplasmic specializations (ES) found at two different level of the seminiferous epithelium. We also used freeze fracture to analyze the characteristics of tubular bulbar complexes (TBC), a known component of apical ectoplasmic specializations. These different approaches also allowed us to study the complex arrangement of the actin cytoskeleton of Sertoli cells branches, which surround germ cells in different stages of the spermatogenic cycle. Our results show a consistent labelling for â-actin before, during and after the release of spermatozoa in the tubular lumen (spermiation) suggesting a significant role of the actin network in spermatic cell differentiation. In conclusion, significant interrelations among the â-actin network, the junctional complexes of the BTB and the ectoplasmic specializations were detected at different stages of the seminiferous cycle.
Palabras clave:
TESTIS
,
SERTOLI CELL
,
ACTIN FILAMENT
,
BETA-ACTIN
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Capítulos de libros(IHEM)
Capítulos de libros de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Capítulos de libros de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Losinno, Antonella Denise; Lopez, Luis Alberto; Capani, Francisco; Foscolo, Mabel Rosa; Ibañez, Jorge Ernesto; et al.; Beta actin: its implication in the seminiferous tubule; Nova Science Publishers, Inc.; 2012; 93-109
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