Artículo

Culturing rat whole ovary for UV filter benzophenone 3 treatment

Santamaría, Clarisa GuillerminaIcon ; Abud, Julián ElíasIcon ; Luque, Enrique HugoIcon ; Kass, LauraIcon ; Rodriguez, Horacio AdolfoIcon
Fecha de publicación: 10/2019
Editorial: John Wiley & Sons, Inc
Revista: Current Protocols in Toxicology
ISSN: 1934-9262
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

Here, we described a detailed protocol to establish a newborn rat whole ovary culture, able to study direct effects (independent of hypothalamic-pituitary-gonadal axis) of Endocrine Disrupting Chemicals (EDC), such as Benzophenone 3 (BP-3). This method is useful to understand changes in follicle formation, primordial to primary transition and expression of regulatory molecules linked to these processes as also provides an alternative to animal models. Basic protocols described here are the following:BASIC PROTOCOL 1: RAT OVARIAN SURGERYBASIC PROTOCOL 2: WHOLE ORGAN/OVARIAN CULTUREBASIC PROTOCOL 3: RNA ISOLATION AND QUANTITATIVE REAL TIME-PCRBASIC PROTOCOL 4: HISTOLOGICAL PROCESSING AND STAINING
Palabras clave: OVARY , RAT , IN VITRO , ORGAN CULTURE
 
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Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/107122
DOI: http://dx.doi.org/10.1002/cptx.89
Colecciones
Articulos(ISAL)
Articulos de INSTITUTO DE SALUD Y AMBIENTE DEL LITORAL
Citación
Santamaría, Clarisa Guillermina; Abud, Julián Elías; Luque, Enrique Hugo; Kass, Laura; Rodriguez, Horacio Adolfo; Culturing rat whole ovary for UV filter benzophenone 3 treatment; John Wiley & Sons, Inc; Current Protocols in Toxicology; 10-2019; 1-29
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