Capítulo de Libro
Imaging of calcium dynamics in pollen Tube cytoplasm
Título del libro: Plant cell expansion: Methods and Protocols
Fecha de publicación:
2015
Editorial:
Springer
ISBN:
978-1-4939-1902-4
Idioma:
Inglés
Clasificación temática:
Resumen
Cytoplasmic calcium [(Ca(2+))cyt] is a central component of cellular signal transduction pathways. In plants, many external and internal stimuli transiently elevate (Ca(2+))cyt, initiating downstream responses that control different features of plant development. In pollen tubes the establishment of an oscillatory gradient of calcium at the tip is essential for polarized growth. Disruption of the cytosolic Ca(2+) gradient by chelators or channel blockers inhibits pollen tube growth. To quantify the physiological role of (Ca(2+))cyt in cellular systems, genetically encoded Ca(2+) indicators such as Yellow Cameleons (YCs) have been developed. The Cameleons are based on a fluorescence resonance energy transfer (FRET) process. Here, we describe a method for imaging cytoplasmic Ca(2+) dynamics in growing pollen tubes that express the fluorescent calcium indicator Yellow Cameleon 3.6 (YC 3.6), using laser-scanning confocal microscopy
Palabras clave:
POLLEN
,
CALCIUM
,
YELLOWCAMELEON 3.6
,
RATIO IMAGING
,
KYMOGRAPH
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Capítulos de libros(INGEBI)
Capítulos de libros de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Capítulos de libros de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
Barberini, María Laura; Muschietti, Jorge Prometeo; Imaging of calcium dynamics in pollen Tube cytoplasm; Springer; 1242; 2015; 49-57
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