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dc.contributor.author
Manavella, Pablo Andrés
dc.contributor.author
Arce, Agustín Lucas
dc.contributor.author
Dezar, Carlos Alberto Alejandro
dc.contributor.author
Bitton, Frédérique
dc.contributor.author
Renou, Jean-Pierre
dc.contributor.author
Crespi, Martin
dc.contributor.author
Chan, Raquel Lia
dc.date.available
2020-06-03T12:40:05Z
dc.date.issued
2006-10
dc.identifier.citation
Manavella, Pablo Andrés; Arce, Agustín Lucas; Dezar, Carlos Alberto Alejandro; Bitton, Frédérique; Renou, Jean-Pierre; et al.; Cross-talk between ethylene and drought signalling pathways is mediated by the sunflower Hahb-4 transcription factor; Wiley Blackwell Publishing, Inc; Plant Journal; 48; 1; 10-2006; 125-137
dc.identifier.issn
0960-7412
dc.identifier.uri
http://hdl.handle.net/11336/106560
dc.description.abstract
Hahb-4 is a member of the Helianthusannuus (sunflower) subfamily I of HD-Zip proteins that is transcriptionally regulated by water availability and abscisic acid. Transgenic Arabidopsis thaliana plants overexpressing this transcription factor (TF) exhibit a characteristic phenotype that includes a strong tolerance to water stress. Here we show that this TF is a new component of ethylene signalling pathways, and that it induces a marked delay in senescence. Plants overexpressing Hahb-4 are less sensitive to external ethylene, enter the senescence pathway later and do not show the typical triple response. Furthermore, transgenic plants expressing this gene under the control of its own inducible promoter showed an inverse correlation between ethylene sensitivity and Hahb-4 levels. Potential targets of Hahb-4 were identified by comparing the transcriptome of Hahb-4-transformed and wild-type plants using microarrays and quantitative RT-PCR. Expression of this TF has a major repressive effect on genes related to ethylene synthesis, such as ACO and SAM, and on genes related to ethylene signalling, such as ERF2 and ERF5. Expression studies in sunflower indicate that Hahb-4 transcript levels are elevated in mature/senescent leaves. Expression of Hahb-4 is induced by ethylene, concomitantly with several genes homologous to the targets identified in the transcriptome analysis (HA-ACOa and HA-ACOb). Transient transformation of sunflower leaves demonstrated the action of Hahb-4 in the regulation of ethylene-related genes. We propose that Hahb-4 is involved in a novel conserved mechanism related to ethylene-mediated senescence that functions to improve desiccation tolerance.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
HaHB4
dc.subject
HD-Zip
dc.subject
Etileno
dc.subject
Tolerancia a sequía
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Cross-talk between ethylene and drought signalling pathways is mediated by the sunflower Hahb-4 transcription factor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-06-01T13:41:34Z
dc.journal.volume
48
dc.journal.number
1
dc.journal.pagination
125-137
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
dc.description.fil
Fil: Bitton, Frédérique. Unite de Recherche en Genomique Vegetale; Francia
dc.description.fil
Fil: Renou, Jean-Pierre. Unite de Recherche en Genomique Vegetale; Francia
dc.description.fil
Fil: Crespi, Martin. Institut des Sciences Vegetales; Francia
dc.description.fil
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.journal.title
Plant Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-313X.2006.02865.x
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