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dc.contributor.author
Coleman, Danielle N.
dc.contributor.author
Carranza Martin, Ana Cristina
dc.contributor.author
Jin, Yukun
dc.contributor.author
Lee, Kichoon
dc.contributor.author
Relling, Alejandro Enrique
dc.date.available
2020-05-27T18:44:57Z
dc.date.issued
2019-05-07
dc.identifier.citation
Coleman, Danielle N.; Carranza Martin, Ana Cristina; Jin, Yukun; Lee, Kichoon; Relling, Alejandro Enrique; Prepartum fatty acid supplementation in sheep: IV. Effect of calcium salts with eicosapentaenoic acid and docosahexaenoic acid in the maternal and finishing diet on lamb liver and adipose tissue during the lamb finishing period; American Society of Animal Science; Journal of Animal Science; 97; 7; 7-5-2019; 3071-3088
dc.identifier.issn
0021-8812
dc.identifier.uri
http://hdl.handle.net/11336/106041
dc.description.abstract
The objective of this study was to evaluate the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplementation to ewes during late gestation on finishing lamb liver and adipose tissue fatty acid (FA) profile and gene expression. Lambs born from ewes supplemented with Ca salts of EPA + DHA, or palm FA distillate (PFAD) high in palmitic and oleic acid at 0.39% DM during the last 50 d of gestation were used. Lambs were weaned at 61 d of age and adapted to a high concentrate diet for 1.5 mo. After adaptation, 74 lambs (28 pens) were blocked by sex and BW and used in a 2 × 2 factorial arrangement of treatments using the factors of dam supplementation (DS) and lamb supplementation (LS) of Ca salts of EPA + DHA or PFAD at 1.48% DM. Lambs were slaughtered after 42 d and liver and adipose tissue collected for FA and gene expression analysis. Liver concentrations of EPA and DHA were greater (P < 0.01) with LS of EPA + DHA vs. PFAD during the finishing period. In adipose tissue, a lamb × dam interaction was observed for EPA (P = 0.02) and DHA (P = 0.04); LS of EPA + DHA increased EPA and DHA, but the increase was greatest in lambs born from ewes supplemented with PFAD. No lamb × dam treatment interactions were observed for gene expression in liver tissue (P > 0.10). Hepatic mRNA abundance of hormone-sensitive lipase (HSL; P = 0.01) was greater in lambs born from EPA + DHA ewes vs. lambs from PFAD ewes. mRNA expression of stearoyl-CoA desaturase (P < 0.01), fatty acid synthase (P = 0.01), Δ5-desaturase (P < 0.01), and Δ6-desaturase (P < 0.01) were decreased in liver of EPA + DHA lambs. A significant lamb × dam diet interaction was observed for elongation of very long chain fatty acid 2 in adipose tissue (P = 0.01); lambs supplemented with the same FA as their dams had lower expression. Expression of HSL tended (P = 0.08) to be decreased in adipose of EPA + DHA lambs born from EPA + DHA ewes. The changes in mRNA expression suggest that lipogenesis decreased, and lipolysis increased in lamb liver with EPA + DHA vs. PFAD supplementation during the finishing period. In adipose tissue, changes suggest that lipogenesis decreased in lambs born from EPA + DHA supplemented dams and supplemented with EPA + DHA during the finishing period. In addition, these results suggest an interaction between supplementation of FA to dams during late gestation on lamb response of adipose tissue, but not liver, to FA supplementation during the finishing period.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Animal Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ADIPOSE TISSUE
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FATTY ACIDS
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FETAL PROGRAMMING
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GENE EXPRESSION
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LAMBS
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LIVER
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Otras Producción Animal y Lechería
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Producción Animal y Lechería
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CIENCIAS AGRÍCOLAS
dc.title
Prepartum fatty acid supplementation in sheep: IV. Effect of calcium salts with eicosapentaenoic acid and docosahexaenoic acid in the maternal and finishing diet on lamb liver and adipose tissue during the lamb finishing period
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-22T15:27:55Z
dc.journal.volume
97
dc.journal.number
7
dc.journal.pagination
3071-3088
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Illinois
dc.description.fil
Fil: Coleman, Danielle N.. Ohio State University; Estados Unidos. University of Illinois at Urbana; Estados Unidos
dc.description.fil
Fil: Carranza Martin, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil
Fil: Jin, Yukun. The College of Wooster; Estados Unidos
dc.description.fil
Fil: Lee, Kichoon. Ohio State University; Estados Unidos
dc.description.fil
Fil: Relling, Alejandro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ohio State University; Estados Unidos
dc.journal.title
Journal of Animal Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jas/article-abstract/97/7/3071/5486460
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jas/skz154
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