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dc.contributor.author
Kayali, Ayse G
dc.contributor.author
Flores, Luis Emilio
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dc.contributor.author
Lopez, Ana D.
dc.contributor.author
Kutlu, Burak
dc.contributor.author
Baetge, Emmanuel
dc.contributor.author
Kitamura, Ryuichi
dc.contributor.author
Hao, Ergeng
dc.contributor.author
Beattie, Gillian M.
dc.contributor.author
Hayek, Alberto
dc.date.available
2020-05-27T18:30:33Z
dc.date.issued
2007-03
dc.identifier.citation
Kayali, Ayse G; Flores, Luis Emilio; Lopez, Ana D.; Kutlu, Burak; Baetge, Emmanuel; et al.; Limited Capacity of Human Adult Islets Expanded In Vitro to Redifferentiate Into Insulin-Producing β-cells; American Diabetes Association; Diabetes; 56; 3; 3-2007; 703-708
dc.identifier.issn
0012-1797
dc.identifier.uri
http://hdl.handle.net/11336/106037
dc.description.abstract
Limited organ availability is an obstacle to the widespread use of islet transplantation in type 1 diabetic patients. To address this problem, many studies have explored methods for expanding functional human islets in vitro for diabetes cell therapy. We previously showed that islet cells replicate after monolayer formation under the influence of hepatocyte growth factor and selected extracellular matrices. However, under these conditions, senescence and loss of insulin expression occur after >15 doublings. In contrast, other groups have reported that islet cells expanded in monolayers for months progressed through a reversible epithelial-to-mesenchymal transition, and that on removal of serum from the cultures, islet-like structures producing insulin were formed (1). The aim of the current study was to compare the two methods for islet expansion using immunostaining, real-time quantitative PCR, and microarrays at the following time points: on arrival, after monolayer expansion, and after 1 week in serum-free media. At this time, cell aliquots were grafted into nude mice to study in vivo function. The two methods showed similar results in islet cell expansion. Attempts at cell differentiation after expansion by both methods failed to consistently recover a β-cell phenotype. Redifferentiation of β-cells after expansion is still a challenge in need of a solution.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Diabetes Association
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ISLETS
dc.subject
REDIFFERETIATION
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INSULIN PRODUCING CELLS
dc.subject.classification
Endocrinología y Metabolismo
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dc.subject.classification
Medicina Clínica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Limited Capacity of Human Adult Islets Expanded In Vitro to Redifferentiate Into Insulin-Producing β-cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-11T15:18:51Z
dc.identifier.eissn
1939-327X
dc.journal.volume
56
dc.journal.number
3
dc.journal.pagination
703-708
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Baltimore
dc.description.fil
Fil: Kayali, Ayse G. University of California at San Diego; Estados Unidos
dc.description.fil
Fil: Flores, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. University of California at San Diego; Estados Unidos
dc.description.fil
Fil: Lopez, Ana D.. University of California at San Diego; Estados Unidos
dc.description.fil
Fil: Kutlu, Burak. Institute for Systems Biology; Estados Unidos
dc.description.fil
Fil: Baetge, Emmanuel. Novocell; Estados Unidos
dc.description.fil
Fil: Kitamura, Ryuichi. University of California at San Diego; Estados Unidos
dc.description.fil
Fil: Hao, Ergeng. University of California at San Diego; Estados Unidos
dc.description.fil
Fil: Beattie, Gillian M.. University of California at San Diego; Estados Unidos
dc.description.fil
Fil: Hayek, Alberto. University of California at San Diego; Estados Unidos
dc.journal.title
Diabetes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://diabetes.diabetesjournals.org/content/56/3/703
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2337/db06-1545
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