Artículo
Molecular subtyping and clonal relatedness of human and cattle verotoxin-producing Escherichia coli O157:H7 isolates
Fecha de publicación:
08/2020
Editorial:
Academic Press Ltd - Elsevier Science Ltd
Revista:
Microbial Pathogenesis
ISSN:
0882-4010
e-ISSN:
1096-1208
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Verotoxin-producing Escherichia coli O157:H7 is the dominant serotype isolated from patients with hemolytic-uremic syndrome (HUS) and, Argentina has the highest rate of HUS in the world. However, not all O157:H7 isolates have the same ability to infect and cause disease in humans. It has been postulated that O157:H7 strains integrate subpopulations related to the origin and virulence. In order to study the population structure and genetic diversity of VTEC O157:H7 from Argentina, a combination of molecular subtyping methods such as multiple loci VNTR analysis (MLVA), single nucleotide polymorphisms (SNP) and phylogroups assignment were used. According to MLVA, high genetic diversity was found among strains isolated from cattle, humans and food. On the other hand, 92% of the isolates presented the allele tir 255 T>A T and 95% were assigned to phylogroup E. We did not find a significant association between the isolates origin and the allele T presence (P>0,05) postulated as significantly overrepresented in human isolates. Our results show that human and cattle VTEC O157:H7 isolates from Argentina are a phylogenetically homogeneous group and, although it presents high genetic diversity in relation to their MLVA and virulence profiles, it is not possible to distinguish divergent populations. The presence in all the strains of a high number of T3SS effectors genes and the no association of phylogenetic subtypes with strain source, is an alert about the potential risk in public health that VTEC O157:H7 cattle strains possess and, at less, a partial explication about the high incidence of HUS in Argentina.
Palabras clave:
ARGENTINA
,
MLVA
,
PHYLOGROUPS ASSIGNMENT
,
SNP
,
VTEC O157:H7
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Citación
González, Juliana; Cadona, Jimena Soledad; Sanso, Andrea Mariel; Bustamante, Ana Victoria; Molecular subtyping and clonal relatedness of human and cattle verotoxin-producing Escherichia coli O157:H7 isolates; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 145; 8-2020; 1-5
Compartir
Altmétricas