Mostrar el registro sencillo del ítem

dc.contributor.author
Sternlieb, Tamara  
dc.contributor.author
Schoijet, Alejandra Cecilia  
dc.contributor.author
Alonso, Guillermo Daniel  
dc.date.available
2020-05-22T19:59:30Z  
dc.date.issued
2020-02  
dc.identifier.citation
Sternlieb, Tamara; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Intracellular cyclic AMP levels modulate differential adaptive responses on epimastigotes and cell culture trypomastigotes of Trypanosoma cruzi; Elsevier Science; Acta Tropica; 202; 105273; 2-2020; 1-8  
dc.identifier.issn
0001-706X  
dc.identifier.uri
http://hdl.handle.net/11336/105799  
dc.description.abstract
Among the many environmental challenges the parasiteTrypanosoma cruzihas to overcome to complete its lifecycle through different hosts, oxidative stress plays a central role. Different stages of this parasite encounterdistinct sources of oxidative stress, such as the oxidative burst of the immune system, or the Heme released fromhemoglobin degradation in the triatomine´s midgut. Also, the redox status of the surroundings functions as asignal to the parasite, triggering processes coupled to differentiation or proliferation. Intracellular secondmessengers, like cAMP, are responsible for the transduction of environmental queues and initiating cellularprocesses accordingly. In trypanosomatids cAMP is involved in a variety of processes, including proliferation,differentiation, osmoregulation and quorum sensing. Trypanosomatid phosphodiesterases (PDE) show atypicalpharmacological properties and some have been involved in key processes for the survival of the parasites, whichvalidates them as attractive therapeutic targets. Our work here shows that cAMP modulates different processesaccording to parasite stage. Epimastigotes become more resistant to oxidative stress when pre-treated with cAMPanalogs, while in trypomastigotes an increase in intracellular cAMP doesn´t seem to aid in this response, althoughit does increase the number of amastigotes obtained 48 h after infection, compared to the control group. Also, weshow that TcrPDEA1, a functionally enigmatic phosphodiesterase with very high Km, is involved in the epi-mastigotes response to oxidative stress.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
CAMP PHOSPHODIESTERASE  
dc.subject
OXIDATIVE STRESS  
dc.subject
SIGNAL TRANSDUCTION  
dc.subject
CHAGAS DISEASE  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Intracellular cyclic AMP levels modulate differential adaptive responses on epimastigotes and cell culture trypomastigotes of Trypanosoma cruzi  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-05-19T19:41:20Z  
dc.identifier.eissn
1873-6254  
dc.journal.volume
202  
dc.journal.number
105273  
dc.journal.pagination
1-8  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Sternlieb, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.description.fil
Fil: Schoijet, Alejandra Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.description.fil
Fil: Alonso, Guillermo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina  
dc.journal.title
Acta Tropica  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0001706X19308563  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2019.105273