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dc.contributor.author
Sternlieb, Tamara
dc.contributor.author
Schoijet, Alejandra Cecilia
dc.contributor.author
Alonso, Guillermo Daniel
dc.date.available
2020-05-22T19:59:30Z
dc.date.issued
2020-02
dc.identifier.citation
Sternlieb, Tamara; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Intracellular cyclic AMP levels modulate differential adaptive responses on epimastigotes and cell culture trypomastigotes of Trypanosoma cruzi; Elsevier Science; Acta Tropica; 202; 105273; 2-2020; 1-8
dc.identifier.issn
0001-706X
dc.identifier.uri
http://hdl.handle.net/11336/105799
dc.description.abstract
Among the many environmental challenges the parasiteTrypanosoma cruzihas to overcome to complete its lifecycle through different hosts, oxidative stress plays a central role. Different stages of this parasite encounterdistinct sources of oxidative stress, such as the oxidative burst of the immune system, or the Heme released fromhemoglobin degradation in the triatomine´s midgut. Also, the redox status of the surroundings functions as asignal to the parasite, triggering processes coupled to differentiation or proliferation. Intracellular secondmessengers, like cAMP, are responsible for the transduction of environmental queues and initiating cellularprocesses accordingly. In trypanosomatids cAMP is involved in a variety of processes, including proliferation,differentiation, osmoregulation and quorum sensing. Trypanosomatid phosphodiesterases (PDE) show atypicalpharmacological properties and some have been involved in key processes for the survival of the parasites, whichvalidates them as attractive therapeutic targets. Our work here shows that cAMP modulates different processesaccording to parasite stage. Epimastigotes become more resistant to oxidative stress when pre-treated with cAMPanalogs, while in trypomastigotes an increase in intracellular cAMP doesn´t seem to aid in this response, althoughit does increase the number of amastigotes obtained 48 h after infection, compared to the control group. Also, weshow that TcrPDEA1, a functionally enigmatic phosphodiesterase with very high Km, is involved in the epi-mastigotes response to oxidative stress.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CAMP PHOSPHODIESTERASE
dc.subject
OXIDATIVE STRESS
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SIGNAL TRANSDUCTION
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CHAGAS DISEASE
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Intracellular cyclic AMP levels modulate differential adaptive responses on epimastigotes and cell culture trypomastigotes of Trypanosoma cruzi
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-19T19:41:20Z
dc.identifier.eissn
1873-6254
dc.journal.volume
202
dc.journal.number
105273
dc.journal.pagination
1-8
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Sternlieb, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Schoijet, Alejandra Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Alonso, Guillermo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.journal.title
Acta Tropica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0001706X19308563
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.actatropica.2019.105273
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