Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Recombinant peptide reverses cryo-capacitation in ram sperm and improves in vitro fertilization

Ledesma, AlbaIcon ; Zalazar, LuciaIcon ; Buchelly Imbachí, Francisco JavierIcon ; Pastore, Juan IgnacioIcon ; Brown, Paula; Eddy, Edward Mitch; Hozbor, Federico Andrés; Cesari, AndreinaIcon
Fecha de publicación: 08/2019
Editorial: Elsevier Science
Revista: Animal Reproduction Science
ISSN: 0378-4320
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Reproductiva

Resumen

Semen cryopreservation is a very important technique for assisted reproduction; however, the cryopreservation process is harmful because it results in a reduction in sperm motility and viability, and leads to premature signals of capacitation, resulting in lesser than desirable fertility rates after artificial insemination. A fraction of seminal plasma, enriched in proteins that contain type II fibronectin domains (FNII) can reverse molecular indicators of cryo-capacitation. The beneficial effects of these proteins, however, depend on the relative abundance in seminal plasma. To create a safe additive for improving frozen sperm functionality, in the present study there was cloning and expression of a recombinant peptide containing four FNII domains (named TrxA-FNIIx4-His6) and evaluation of its effect after addition to frozen/thawed ram sperm. The cDNA for this protein was expressed in E. coli and after denaturation and re-naturalization of the protein, toxicity and binding capacity were assessed. By fluorescent labelling assessment, there was binding of the protein to the thawed sperm. At the two doses used (0.15 and 0.3 μM), TrxA-FNIIx4-His6 had the capacity to reverse the molecular indicators of cryo-capacitation as indicated by the reduction on phosphorylated substrates of PKA. Furthermore, the supplementation with this protein resulted in a normal capacitation process as evidenced by the increase in the in vitro fertilization rate when the greatest concentration of the protein was evaluated (73.25 ± 2.95; 40.13 ± 11.82 for 0.3 μM and control, respectively). There was no effect of protein supplementation on sperm objective motility compared to untreated sperm. In conclusion, the use of TrxA-FNIIx4-His6 is a promising biotechnological approach for cryopreserving ram sperm and maintaining sperm viability.
Palabras clave: CRYOPRESERVATION , FIBRONECTIN (FNII) DOMAINS , RAM , RECOMBINANT PEPTIDE , SEMINAL PLASMA
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 956.8Kb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/105426
URL: https://www.sciencedirect.com/science/article/abs/pii/S0378432018311138?via%3Dih
DOI: https://doi.org/10.1016/j.anireprosci.2019.05.016
Colecciones
Articulos(ICYTE)
Articulos de INSTITUTO DE INVESTIGACIONES CIENTIFICAS Y TECNOLOGICAS EN ELECTRONICA
Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Ledesma, Alba; Zalazar, Lucia; Buchelly Imbachí, Francisco Javier; Pastore, Juan Ignacio; Brown, Paula; et al.; Recombinant peptide reverses cryo-capacitation in ram sperm and improves in vitro fertilization; Elsevier Science; Animal Reproduction Science; 207; 8-2019; 61-72
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES