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dc.contributor.author
Lizarraga, Maria Alfonsina
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dc.contributor.author
Posik, Diego Manuel
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dc.contributor.author
Zappa, María Eugenia
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dc.contributor.author
Castillo, Nadia Sabiela
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dc.contributor.author
Giovambattista, Guillermo
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dc.date.available
2020-05-18T20:18:35Z
dc.date.issued
2019-12-02
dc.identifier.citation
Lizarraga, Maria Alfonsina; Posik, Diego Manuel; Zappa, María Eugenia; Castillo, Nadia Sabiela; Giovambattista, Guillermo; Experimentation with Animals: A Key Aspect of the 3Rs. The Genetic Quality; Acta Scientific; Acta Scientific Microbiology; 3; 1; 2-12-2019; 62-65
dc.identifier.issn
2581-3226
dc.identifier.uri
http://hdl.handle.net/11336/105415
dc.description.abstract
The genetic quality of laboratory animals is essential for reproducibility of scientific research. Working with animals of certifiedgenetic quality is still a pending issue in Argentina due to the lack of routine genetic controls, of information on the genetic background of animals and of proper training. Apart from being concerned with having their results published and getting funding for research, scientists should know the genetic origin of laboratory animals. Consequently, they should perform genetic controls to verifywhether animal integrity has been compromised by accidental genetic contamination or genetic drift. The aim of this work was toevaluate the genetic purity of the inbred C57BL/6J mouse strain from three animal facilities belonging to the Buenos Aires UniversitySchool of Medicine network by analyzing a panel of microsatellite markers. Female mice tail samples (3-5 mm) were taken and genomic DNA was obtained by organic extraction. The genetic profile of each animal was determined by PCR-fragment analysis, usingmicrosatellites D1Mit155, D2Mit493, D3Mit49, D13Mit13, D6Mit8 and D12Mit12, located on six different autosomal chromosomesand selected from the Mouse Genome Informatics database (www.informatics.jax.org/searches). The results obtained provided keydata on the genetic quality of the three inbred animal colonies studied. They also served as an example for other laboratory animalfacilities in Argentina and as a starting point to modify the conditions and management of laboratory animal colonies. We determinedthe genetic purity of the inbred C57BL/6J mouse strain in all animal facilities evaluated. All six loci analyzed were homozygous,certifying their isogenicity and phenotypic uniformity. These results are promising for animal facilities mainly performing biomedical research. They also show a positive evolution in handling animal colonies and use of the 3Rs, and researcher commitment withanimal science, since they promote the supply of genetically quality-controlled animals. The positive impact of these results shouldencourage other researchers using this inbred strain to perform periodic genetic monitoring, thereby consolidating the supply ofquality-controlled mice. This pioneering study carried out in IGEVET (CONICET- UNLP) should consolidate the genetic monitoring ofinbred strains throughout the country.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Acta Scientific
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
3Rs
dc.subject
C57BL/6J
dc.subject
Genetic Monitoring
dc.subject
Conventional Animal Care Facility
dc.subject.classification
Otras Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Experimentation with Animals: A Key Aspect of the 3Rs. The Genetic Quality
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-22T15:26:51Z
dc.journal.volume
3
dc.journal.number
1
dc.journal.pagination
62-65
dc.journal.pais
India
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dc.journal.ciudad
Hyderabad
dc.description.fil
Fil: Lizarraga, Maria Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil
Fil: Posik, Diego Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil
Fil: Zappa, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil
Fil: Castillo, Nadia Sabiela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.journal.title
Acta Scientific Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://actascientific.com/ASMI/experimentation-with-animals-a-key-aspect-of-the-3rs-the-genetic-quality.php
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.31080/ASMI.2020.03.experimentation-with-animals-a-key-aspect-of-the-3rs-the-genetic-quality
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