Artículo
PTHrP treatment of colon cancer cells promotes tumor associated-angiogenesis by the effect of VEGF
Calvo, Natalia Graciela
; Carriere, Pedro Matias
; Martín, María Julia
; Gigola, Graciela; Gentili, Claudia Rosana
Fecha de publicación:
03/2019
Editorial:
Elsevier Ireland
Revista:
Molecular and Cellular Endocrinology
ISSN:
0303-7207
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We showed that Parathyroid Hormone-related Peptide (PTHrP) induces proliferation, migration, survival and chemoresistance via MAPKs and PI3K/AKT pathways in colorectal cancer (CRC) cells. The objective of this study was to investigate if PTHrP is also involved in tumor angiogenesis. PTHrP increased VEGF expression and the number of structures with characteristics of neoformed vessels in xenografts tumor. Also, PTHrP increased mRNA levels of VEGF, HIF-1α and MMP-9 via ERK1/2 and PI3K/Akt pathways in Caco-2 and HCT116 cells. Tumor conditioned media (TCMs) from both cell lines treated with PTHrP increases the number of cells, the migration and the tube formation in the endothelial HMEC-1 cells, whereas the neutralizing antibody against VEGF diminished this response. In contrast, PTHrP by direct treatment only increased ERK1/2 phosphorylation and the HMEC-1 cells number. These results provide the first evidence related to the mode of action of PTHrP that leads to its proangiogenic effects in the CRC.
Palabras clave:
PTHrP
,
COLON CANCER CELLS
,
ANGIOGENESIS
,
VEGF
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Articulos(INBIOSUR)
Articulos de INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Articulos de INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Articulos(INQUISUR)
Articulos de INST.DE QUIMICA DEL SUR
Articulos de INST.DE QUIMICA DEL SUR
Citación
Calvo, Natalia Graciela; Carriere, Pedro Matias; Martín, María Julia; Gigola, Graciela; Gentili, Claudia Rosana; PTHrP treatment of colon cancer cells promotes tumor associated-angiogenesis by the effect of VEGF; Elsevier Ireland; Molecular and Cellular Endocrinology; 483; 3-2019; 50-63
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