Artículo
Accessibility of promoter DNA is not the primary determinant of chromatin-mediated gene regulation
Fecha de publicación:
08/2019
Editorial:
Cold Spring Harbor Laboratory Press
Revista:
Genome Research
ISSN:
1088-9051
e-ISSN:
1549-5469
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
DNA accessibility is thought to be of major importance in regulating gene expression. We test this hypothesis using a restriction enzyme as a probe of chromatin structure and as a proxy for transcription factors. We measured the digestion rate and the fraction of accessible DNA at almost all genomic AluI sites in budding yeast and mouse liver nuclei. Hepatocyte DNA is more accessible than yeast DNA, consistent with longer linkers between nucleosomes, and suggesting that nucleosome spacing is a major determinant of accessibility. DNA accessibility varies from cell to cell, such that essentially no sites are accessible or inaccessible in every cell. AluI sites in inactive mouse promoters are accessible in some cells, implying that transcription factors could bind without activating the gene. Euchromatin and heterochromatin have very similar accessibilities, suggesting that transcription factors can penetrate heterochromatin. Thus, DNA accessibility is not likely to be the primary determinant of gene regulation.
Palabras clave:
CHROMATIN
,
ACCESSIBILITY
,
RESTRICTION ENZYME
,
GENE REGULATION
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Articulos(INGEBI)
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Articulos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
Chereji, Razvan V.; Eriksson, Peter R.; Ocampo, Josefina; Prajapati, Hemant Kumar; Clark, David; Accessibility of promoter DNA is not the primary determinant of chromatin-mediated gene regulation; Cold Spring Harbor Laboratory Press; Genome Research; 29; 12; 8-2019; 1985-1995
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