Artículo
Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples
Marin Barroso, Evelyn del Valle
; Messina, Germán Alejandro
; Bertolino, Franco Adrián
; Raba, Julio
; Pereira, Sirley Vanesa
Fecha de publicación:
04/2019
Editorial:
Royal Society of Chemistry
Revista:
Analytical Methods
ISSN:
1759-9660
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The gluten-free diet is a unique, effective treatment for different conditions related to gluten consumption. Therefore, it is crucial the availability of new methodologies for the sensitive and specific determination of gluten content in food samples. Herein, a screen printed electrode modified with carbon nanofibers coupled to a paper immunoaffinity platform was reported for the determination of gliadin in foods samples. The paper microzone covalently functionalized with specific anti-gliadin antibodies was placed on the modified electrode surface for the electrochemical determination of gliadin. The surface of the electrode modified with carbon nanofibers was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), which showed the improved sensitivity of the modified surface. The developed device was evaluated using different flour samples obtaining a favorable response. The calculated limit of detection for the device in analyzed samples was 0.005 mg kg -1 and for the enzyme-linked immunosorbent assay was 1.5 mg kg -1 . The coefficient of variation (CV) for the determination of 20 μg kg -1 of gliadin was 4.11%. The disposable electrochemical sensor developed, represents an easy-to-use and low-cost strategy for the determination of gliadin in food samples.
Palabras clave:
GLIADIN
,
IMMUNOSENSOR
,
ELECTROCHEMISTRY
,
CARBON NANOFIBER
,
FOOD
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Identificadores
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Marin Barroso, Evelyn del Valle; Messina, Germán Alejandro; Bertolino, Franco Adrián; Raba, Julio; Pereira, Sirley Vanesa; Electrochemical immunosensor modified with carbon nanofibers coupled to a paper platform for the determination of gliadins in food samples; Royal Society of Chemistry; Analytical Methods; 11; 16; 4-2019; 2170-2178
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