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dc.contributor.author
Valva, Pamela
dc.contributor.author
Gismondi, Maria Ines
dc.contributor.author
Casciato, Paola
dc.contributor.author
Galoppo, Marcela
dc.contributor.author
Lezama, Carol
dc.contributor.author
Galdame, Omar
dc.contributor.author
Gadano, Adrián Carlos
dc.contributor.author
Galoppo, María Cristina
dc.contributor.author
Mullen, Eduardo
dc.contributor.author
de Matteo, Elena Noemí
dc.contributor.author
Preciado, María Victoria
dc.date.available
2020-05-14T15:36:53Z
dc.date.issued
2014-12
dc.identifier.citation
Valva, Pamela; Gismondi, Maria Ines; Casciato, Paola; Galoppo, Marcela; Lezama, Carol; et al.; Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection; Elsevier; Clinical Microbiology And Infection; 20; 12; 12-2014; 998-1009
dc.identifier.issn
1198-743X
dc.identifier.uri
http://hdl.handle.net/11336/105118
dc.description.abstract
Mechanisms leading to liver damage in chronic hepatitis C (CHC) are being discussed, but both the immune system and the virus are involved. The aim of this study was to evaluate intrahepatic viral infection, apoptosis and portal and periportal/interface infiltrate in paediatric and adult patients to elucidate the pathogenesis of chronic hepatitis C. HCV-infected, activated caspase-3+ and TUNEL+ hepatocytes, as well as total, CD4+, CD8+, Foxp3+ and CD20+ lymphocytes infiltrating portal and periportal/interface tracts were evaluated in 27 paediatric and 32 adult liver samples by immunohistochemistry or immunofluorescence. The number of infected hepatocytes was higher in paediatric than in adult samples (p 0.0078). In children, they correlated with apoptotic hepatocytes (activated caspase-3+r = 0.74, p < 0.0001; TUNEL+r = 0.606, p 0.0017). Also, infected (p = 0.026) and apoptotic hepatocytes (p = 0.03) were associated with the severity of fibrosis. In adults, activated caspase-3+ cell count was increased in severe hepatitis (p = 0.009). Total, CD4+, CD8+ and Foxp3+ lymphocyte count was higher in adult samples (p < 0.05). Paediatric CD8+ cells correlated with infected (r = 0.495, p 0.04) and TUNEL+ hepatocytes (r = 0.474, p = 0.047), while adult ones correlated with activated caspase-3+ hepatocytes (r = 0.387, p 0.04). In adults, CD8+ was associated with hepatitis severity (p < 0.0001) and correlated with inflammatory activity (CD8+ r = 0.639, p 0.0003). HCV, apoptosis and immune response proved to be involved in CHC pathogenesis of both paediatric and adult patients. However, liver injury in paediatric CHC would be largely associated with a viral cytopathic effect mediated by apoptosis, while in adults it would be mainly associated with an exacerbated immune response.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
APOPTOSIS
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HEPATITIS C VIRUS
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INFECTED HEPATOCYTES
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INFILTRATED MICROENVIRONMENT
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PATHOGENESIS
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
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Virología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-05-11T15:08:38Z
dc.journal.volume
20
dc.journal.number
12
dc.journal.pagination
998-1009
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Valva, Pamela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil
Fil: Casciato, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
dc.description.fil
Fil: Galoppo, Marcela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Lezama, Carol. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Galdame, Omar. Hospital Italiano; Argentina
dc.description.fil
Fil: Gadano, Adrián Carlos. Hospital Italiano; Argentina
dc.description.fil
Fil: Galoppo, María Cristina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
dc.description.fil
Fil: Mullen, Eduardo. Hospital Italiano; Argentina
dc.description.fil
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
dc.journal.title
Clinical Microbiology And Infection
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(15)60051-9/fulltext
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/1469-0691.12728
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