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dc.contributor.author
Muñoz, Claudio Javier
dc.contributor.author
Fanzone, Martín Leandro
dc.contributor.author
Lijavetzky, Diego Claudio
dc.date.available
2020-05-12T17:51:06Z
dc.date.issued
2019-11
dc.identifier.citation
Muñoz, Claudio Javier; Fanzone, Martín Leandro; Lijavetzky, Diego Claudio; Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar "Malbec" by putative R2R3 MYB negative regulators; Elsevier Science; Scientia Horticulturae; 257; 11-2019; 1-10
dc.identifier.issn
0304-4238
dc.identifier.uri
http://hdl.handle.net/11336/104900
dc.description.abstract
In grapevine, proanthocyanins (PAs) and anthocyanins are obtained via the phenylpropanoid pathway. These metabolites are synthesized in specific tissues of the berry and accumulate in different developmental stages. The transcriptional control of the route is affected by transcription factors (TFs), mainly R2R3- Myb type, which can act as activators or repressors. Here we studied the fine regulation of the pathway by analysing the expression profiles of nine TFs operating as activators of the route as well as two main structural genes. In addition, we analysed the profiles of six R2R3-Myb subgroup 4 TFs described as repressors of the route (VviMybC2-L1, VviMybC2-L2, VviMybC2-L3, VviMyb4a, VviMyb4b and VviMyb4-like). The analysis of gene expression was carried out in five different development stages of the berry and in two berry tissues (skin and flesh) of cultivar ?Malbec?. On the other hand, the putative R2R3-Myb repressors were analysed and compared in the tenturier cultivar ?Alicante Bouschet?, in the same tissues and stages of development as ?Malbec?. Concerning the R2R3-Myb repressors, our results indicate that in the ?Malbec? skins, VviMyb4a and VviMyb4b would act on the early genes of the route while VviMybC2-L2 and VviMyb4-like are co-expressed towards the end of the anthocyanin biosynthesis. In addition, the VviMybC2-L1 and VviMybC2-L3 are induced at two stages of development, first towards the end of the green phase of the berry, indicating the possible synergistic interaction by repressing the synthesis of PAs, then they are induced again towards maturation to inhibit the synthesis of anthocyanins. On the other hand, our results showed that the expression profiles of Myb repressor genes may explain the presence of colour in the flesh of Alicante Bouschet variety.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MALBEC
dc.subject
TRANSCRIPTOMICA
dc.subject
ANTOCIANINAS
dc.subject
MYB-REPRESSORS
dc.subject.classification
Ciencias de las Plantas, Botánica
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Transcriptional regulation of the anthocyanin biosynthesis pathway in developing grapevine berries in cultivar "Malbec" by putative R2R3 MYB negative regulators
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-28T14:34:36Z
dc.journal.volume
257
dc.journal.pagination
1-10
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.description.fil
Fil: Fanzone, Martín Leandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza. Laboratorio de Aromas y Sustancias Naturales; Argentina
dc.description.fil
Fil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.journal.title
Scientia Horticulturae
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423819305497
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.scienta.2019.108663
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