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Artículo

A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants

Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; Romanò, Cecilia; Previtali, Viola; Almeida, Juliana; Bréhélin, Claire; Asis, RamónIcon ; Quadrana, Leandro DanielIcon ; Demarco, Diego Gabriel; Alseekh, Saleh; Salinas Gamboa, Rigel; Pérez-Flores, Laura; Dominguez, Pia GuadalupeIcon ; Rothan, Christophe; Fernie, Alisdair Robert; González, Maurício; Stocker, Achim; Hemmerle, Andreas; Clausen, Mads Hartving; Carrari, Fernando OscarIcon ; Rossi, Magdalena
Fecha de publicación: 11/2018
Editorial: NLM (Medline)
Revista: Plant & Cell Physiology
ISSN: 1471-9053
e-ISSN: 1471-9053
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.
Palabras clave: TOCOPHEROL , TOMATO , TOCOPHEROL TRANSPORT
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/104898
DOI: http://dx.doi.org/10.1093/pcp/pcy191
URL: https://www.ncbi.nlm.nih.gov/pubmed/30239816
Colecciones
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; et al.; A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants; NLM (Medline); Plant & Cell Physiology; 59; 11; 11-2018; 2188-2203
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