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dc.contributor.author
Pagano, Eleonora
dc.contributor.author
Fontana, Vanina Andrea
dc.contributor.author
Calvo, Juan Carlos
dc.date.available
2016-12-28T17:00:11Z
dc.date.issued
2011-09
dc.identifier.citation
Pagano, Eleonora; Fontana, Vanina Andrea; Calvo, Juan Carlos; Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes; Spandidos Publ Ltd; Molecular Medicine Reports; 4; 5; 9-2011; 955-961
dc.identifier.issn
1791-2997
dc.identifier.uri
http://hdl.handle.net/11336/10474
dc.description.abstract
Previously, we studied the erythroblastic leukemia viral oncogene homolog (erbB) family of tyrosine kinase growth factor receptors in terms of protein expression, modulation and activation in the 3T3-L1 cell line. In the present study, the presence of full-length erbB mRNAs, and splice or proteolytic erbB variants, was evaluated using RT-PCR. Epidermal growth factor receptor (EGFR)/erbB1 expression was confirmed. Wild-type (wt) ErbB2, human erbB2 (HER2)-extracellular domain (ECD) and herstatin mRNA expression were analyzed. Restriction analysis confirmed wt expression. 3T3-L1 cells exhibited HER2-ECD and herstatin mRNA expression, although at extremely low levels (compared to the control cell lines). ErbB3 cDNA was amplified using mouse mammary tumors and rat acines as positive controls. ErbB4 was not positively identified in these cells. In conclusion, this study demonstrates that 3T3-L1 cells express EGFR/erbB1, erbB2 and erbB3, and possibly, certain erbB2 splice or proteolytic variants, which are worth pursuing.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Spandidos Publ Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Erythroblastic Leukemia Viral Oncogene Homolog
dc.subject
Epidermal Growth Factor Receptor
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3t3-L1 Cells
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Preadipocytes
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-12-27T20:13:06Z
dc.identifier.eissn
1791-3004
dc.journal.volume
4
dc.journal.number
5
dc.journal.pagination
955-961
dc.journal.pais
Grecia
dc.journal.ciudad
Atenas
dc.description.fil
Fil: Pagano, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Pontificia Universidad Católica Argentina "Santa María de Los Buenos Aires"; Argentina
dc.description.fil
Fil: Fontana, Vanina Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.journal.title
Molecular Medicine Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/10.3892/mmr.2011.517
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3892/mmr.2011.517
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