Artículo
Oxfendazole kinetics in pigs: In vivo assessment of its pattern of accumulation in Ascaris suum
Ceballos, Laura
; Cantón, Candela
; Cadenazzi, Gabriela Esther; Virkel, Guillermo Leon
; Dominguez, Maria Paula
; Moreno Torrejon, Laura
; Lanusse, Carlos Edmundo
; Alvarez, Luis Ignacio
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
04/2019
Editorial:
Academic Press Inc Elsevier Science
Revista:
Experimental Parasitology
ISSN:
0014-4894
e-ISSN:
1090-2449
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Ascaris suum is a widespread parasitic nematode that causes infection in pigs with high prevalence rates. Oxfendazole (OFZ) is effective against A. suum when used at a single high oral dose of 30 mg/kg. The aim of this study was to assess the pattern of distribution/accumulation of OFZ and its metabolites, in bloodstream (plasma), mucosal tissue and contents from small and large intestine and adult specimens of A. suum collected from infected and treated pigs. The activity of glutathione-S-transferases (GSTs) in A. suum was also investigated. Infected pigs were orally treated with OFZ (30 mg/kg) and sacrificed at 0, 3, 6 and 12 h after treatment. Samples of blood, mucosa and contents from both small and large intestine as well as adult worms were obtained and processed for quantification of OFZ/metabolites by HPLC. OFZ was the main analyte measured in all of the evaluated matrixes. The highest drug concentrations were determined in small (AUC 0-t 718.7 ± 283.5 μg h/g) and large (399.6 ± 110.5 μg h/g) intestinal content. Concentrations ranging from 1.35 to 2.60 μg/g (OFZ) were measured in adult A. suum. GSTs activity was higher after exposure to OFZ both in vivo and ex vivo. The data obtained here suggest that the pattern of OFZ accumulation in A. suum would be more related to the concentration achieved in the fluid and mucosa of the small intestine than in other tissues/fluids. It is expected that increments in the amount of drug attained in the tissues/fluids of parasite location will correlate with increased drug concentration within the target parasite, and therefore with the resultant treatment efficacy. The results are particularly relevant considering the potential of OFZ to be used for soil transmitted helminths (STH) control programs and the advantages of pigs as a model to assess drug treatment to be implemented in humans.
Palabras clave:
ASCARIS SUUM
,
DRUG ACCUMULATION
,
OXFENDAZOLE
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Citación
Ceballos, Laura; Cantón, Candela; Cadenazzi, Gabriela Esther; Virkel, Guillermo Leon; Dominguez, Maria Paula; et al.; Oxfendazole kinetics in pigs: In vivo assessment of its pattern of accumulation in Ascaris suum; Academic Press Inc Elsevier Science; Experimental Parasitology; 199; 4-2019; 52-58
Compartir
Altmétricas