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Artículo

A three-domain copper-nitrite reductase with a unique sensing loop

Opperman, Diederik Johannes; Murgida, Daniel HoracioIcon ; Dalosto, Sergio DanielIcon ; Brondino, Carlos DanteIcon ; Ferroni, Felix MartínIcon
Fecha de publicación: 01/2019
Editorial: International Union of Crystallography
Revista: IUCrJ
ISSN: 2052-2525
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Inorgánica y Nuclear

Resumen

Dissimilatory nitrite reductases are key enzymes in the denitrification pathway, reducing nitrite and leading to the production of gaseous products (NO, N2O and N2). The reaction is catalysed either by a Cu-containing nitrite reductase (NirK) or by a cytochrome cd 1 nitrite reductase (NirS), as the simultaneous presence of the two enzymes has never been detected in the same microorganism. The thermophilic bacterium Thermus scotoductus SA-01 is an exception to this rule, harbouring both genes within a denitrification cluster, which encodes for an atypical NirK. The crystal structure of TsNirK has been determined at 1.63Å resolution. TsNirK is a homotrimer with subunits of 451 residues that contain three copper atoms each. The N-Terminal region possesses a type 2 Cu (T2Cu) and a type 1 Cu (T1CuN) while the C-Terminus contains an extra type 1 Cu (T1CuC) bound within a cupredoxin motif. T1CuN shows an unusual Cu atom coordination (His2-Cys-Gln) compared with T1Cu observed in NirKs reported so far (His2-Cys-Met). T1CuC is buried at ∼5Å from the molecular surface and located ∼14.1Å away from T1CuN; T1CuN and T2Cu are ∼12.6Å apart. All these distances are compatible with an electron-Transfer process T1CuC → T1CuN → T2Cu. T1CuN and T2Cu are connected by a typical Cys-His bridge and an unexpected sensing loop which harbours a SerCAT residue close to T2Cu, suggesting an alternative nitrite-reduction mechanism in these enzymes. Biophysicochemical and functional features of TsNirK are discussed on the basis of X-ray crystallography, electron paramagnetic resonance, resonance Raman and kinetic experiments.
Palabras clave: SER , THERMUS SCOTODUCTUS SA-01 , THREE-DOMAIN COPPER-NITRITE REDUCTASE , X-RAY CRYSTAL STRUCTURE
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/104706
URL: http://scripts.iucr.org/cgi-bin/paper?S2052252519000241
DOI: http://dx.doi.org/10.1107/S2052252519000241
Colecciones
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(INQUIMAE)
Articulos de INST.D/QUIM FIS D/L MATERIALES MEDIOAMB Y ENERGIA
Citación
Opperman, Diederik Johannes; Murgida, Daniel Horacio; Dalosto, Sergio Daniel; Brondino, Carlos Dante; Ferroni, Felix Martín; A three-domain copper-nitrite reductase with a unique sensing loop; International Union of Crystallography; IUCrJ; 6; 2; 1-2019; 248-258
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