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dc.contributor.author
Militello, Rodrigo Damián
dc.contributor.author
Munafo, Daniela B.
dc.contributor.author
Beron, Walter
dc.contributor.author
Lopez, Luis Alberto
dc.contributor.author
Monier, Solange
dc.contributor.author
Goud, Bruno
dc.contributor.author
Colombo, Maria Isabel
dc.date.available
2016-12-28T16:47:38Z
dc.date.issued
2013-03-08
dc.identifier.citation
Militello, Rodrigo Damián; Munafo, Daniela B.; Beron, Walter; Lopez, Luis Alberto; Monier, Solange; et al.; Rab24 is required for normal cell division; Wiley; Traffic; 14; 5; 8-3-2013; 502-518
dc.identifier.issn
1398-9219
dc.identifier.uri
http://hdl.handle.net/11336/10466
dc.description.abstract
Rab24 is an atypical member of the Rab GTPase family whose distribution in interphase cells has been characterized; however, its function remains largely unknown. In this study, we have analyzed the distribution of Rab24 throughout cell division. We have observed that Rab24 was located at the mitotic spindle in metaphase, at the midbody during telophase and in the furrow during cytokinesis. We have also observed partial co-localization of Rab24 and tubulin and demonstrated its association to microtubules. Interestingly, more than 90% of transiently transfected HeLa cells with Rab24 presented abnormal nuclear connections (i.e., chromatin bridges). Furthermore, in CHO cells stably transfected with GFP-Rab24wt, we observed a large percentage of binucleated and multinucleated cells. In addition, these cells presented an extremely large size and multiple failures in mitosis, as aberrant spindle formation (metaphase), delayed chromosomes (telophase) and multiple cytokinesis. A marked increase in binucleated, multinucleated and multilobulated nucleus formation was observed in HeLa cells depleted of Rab24. We also present evidence that a fraction of Rab24 associates with microtubules. In addition, Rab24 knock down resulted in misalignment of chromosomes and abnormal spindle formation in metaphase leading to the appearance of delayed chromosomes during late telophase and failures in cytokinesis. Our findings suggest that an adequate level of Rab24 is necessary for normal cell division. In summary, Rab24 modulates several mitotic events, including chromosome segregation and cytokinesis, perhaps through the interaction with microtubules.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Rab
dc.subject
Rab24
dc.subject
Mitosis
dc.subject
Cell Ciycle
dc.subject
Chromosome Segregation
dc.subject
Cytokinesis
dc.subject
Micro-Tubules
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Rab24 is required for normal cell division
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-09-19T18:45:36Z
dc.journal.volume
14
dc.journal.number
5
dc.journal.pagination
502-518
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Militello, Rodrigo Damián. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: Munafo, Daniela B.. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: Beron, Walter. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: Monier, Solange. Institut Curie. Molecular Mechanisms of Intracellular Transport Laboratoratory; Francia
dc.description.fil
Fil: Goud, Bruno. Institut Curie. Molecular Mechanisms of Intracellular Transport Laboratoratory; Francia
dc.description.fil
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.journal.title
Traffic
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/23387408
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tra.12057/abstract


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