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Artículo

Endometriosis progression in tumor necrosis factor receptor p55-deficient mice: Impact on oxidative/nitrosative stress and metallomic profile

Delsouc, María BelénIcon ; Ghersa, FedericaIcon ; Ramirez, DarioIcon ; Della Vedova, Maria CeciliaIcon ; Gil, Raul AndresIcon ; Vallcaneras, SandraIcon ; Casais, MarilinaIcon
Fecha de publicación: 03/2019
Editorial: Elsevier Gmbh
Revista: Journal of Trace Elements in Medicine and Biology
ISSN: 0946-672X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Médicas

Resumen

The present study was conducted to investigate whether the deficiency of tumor necrosis factor receptor p55 (TNFRp55) modulates oxidative/nitrosative stress and metallomic profile into the peritoneal cavity during the experimental endometriosis progression in mice. Female C57BL/6 mice, wild-type (WT) and TNFRp55 knockout (KO) of two months were used. Endometriosis was induced experimentally by autotransplanting three pieces of the right uterine horn to the intestinal mesentery. After four weeks, endometriotic-like lesions and peritoneal lavage fluid were collected. The obtained peritoneal fluid was analyzed for nitrite levels using the Griess method and trace elements concentrations by ICP-MS. Both endometriotic-like lesions and cells isolated from peritoneal lavage were analyzed for the following oxidative/nitrosative stress markers: inducible nitric oxide synthase (iNOS) expression by Western Blot; total antioxidant capacity (TAC), the activity of two antioxidant enzymes (CAT and GPX) and thiobarbituric acid-reactive substances (TBARS) concentration, by spectrophotometric method; and protein carbonyl content and nitrotyrosine presence by ELISA. In comparison to WT group, KO mice exhibited larger lesion volume; higher levels of nitrite, copper (Cu) and strontium (Sr) in the peritoneal fluid; increased TAC, CAT, and GPX in peritoneal lavage cells; decreased concentration of TBARS in lesions and protein carbonyl in peritoneal lavage cells. Significant positive correlations between Cu and lesion volume, Sr and lesion volume, and Cu and Sr were obtained. Our results suggest that the TNFRp55 deficiency increases antioxidant protection and promotes high Cu-Sr concentrations in the peritoneal cavity, which favors the progression of experimental endometriosis.
Palabras clave: ENDOMETRIOSIS , NITROSATIVE STRESS , OXIDATIVE STRESS , TRACE ELEMENTS , TUMOR NECROSIS FACTOR RECEPTOR P55
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/104429
URL: https://www.sciencedirect.com/science/article/abs/pii/S0946672X18306643
DOI: http://dx.doi.org/10.1016/j.jtemb.2018.12.013
Colecciones
Articulos(IMIBIO-SL)
Articulos de INST. MULTIDICIPLINARIO DE INV. BIO. DE SAN LUIS
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Delsouc, María Belén; Ghersa, Federica; Ramirez, Dario; Della Vedova, Maria Cecilia; Gil, Raul Andres; et al.; Endometriosis progression in tumor necrosis factor receptor p55-deficient mice: Impact on oxidative/nitrosative stress and metallomic profile; Elsevier Gmbh; Journal of Trace Elements in Medicine and Biology; 52; 3-2019; 157-165
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