Artículo
In Vitro and In Vivo Evaluation of Voriconazole-Containing Antifungal Combinations against Mucorales Using a Galleria mellonella Model of Mucormycosis
Macedo, Daiana
; Leonardelli, Florencia
; Dudiuk, Catiana Beatriz
; Vitale, Roxana Gabriela
; del Valle, Eleodoro Eduardo
; Giusiano, Gustavo Emilio
; Gamarra, Soledad; García Effron, Guillermo
Fecha de publicación:
01/2019
Editorial:
Multidisciplinary Digital Publishing Institute
Revista:
Journal of Fungi
e-ISSN:
2309-608X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Mucorales are resistant to most antifungals. Mucormycosis associated mortality is unacceptable and new treatment approaches are needed. The objectives of this work were (i) to evaluate the nature and intensity of the in vitro effect of three drugs combinations which included voriconazole (plus amphotericin B, posaconazole and caspofungin) against 25 strains of six different Mucorales species; (ii) to evaluate a Galleria mellonella mucormycosis model; and (iii) to establish if any in vitro?in vivo correlation exists. As expected, amphotericin B and posaconazole were the most active drugs when tested alone. However, species-specific differences were found. The ΣFICs varied according to the used combination. Only five strains showed synergism when voriconazole was combined with posaconazole and three strains when combined with amphotericin B. Microscopic hyphae alteration were observed for some isolates when confronted against drugs combinations. Using a Galleria mellonella mucormycosis model, better survival was seen in voriconazole plus amphotericin B and plus caspofungin combined treatments when compared with AMB alone for R. microsporus. These survival improvements were obtained using a 32-fold lower amphotericin B doses when combined with VRC than when treated with the polyene alone. These lower antifungal doses emulate the antifungal concentrations where the microscopic hyphae alterations were seen.
Palabras clave:
AMPHOTERICIN B
,
AZOLE
,
GALLERIA MELLONELLA
,
MUCORMYCOSIS
,
SYNERGISM
,
VORICONAZOLE
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - NORDESTE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NORDESTE
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Macedo, Daiana; Leonardelli, Florencia; Dudiuk, Catiana Beatriz; Vitale, Roxana Gabriela; del Valle, Eleodoro Eduardo; et al.; In Vitro and In Vivo Evaluation of Voriconazole-Containing Antifungal Combinations against Mucorales Using a Galleria mellonella Model of Mucormycosis; Multidisciplinary Digital Publishing Institute; Journal of Fungi; 5; 1; 1-2019; 1-12
Compartir
Altmétricas