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dc.contributor.author
Manavella, Pablo Andrés
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dc.contributor.author
Chan, Raquel Lia
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dc.date.available
2020-05-01T12:51:50Z
dc.date.issued
2009-10
dc.identifier.citation
Manavella, Pablo Andrés; Chan, Raquel Lia; Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies; Nature Publishing Group; Nature Protocols; 4; 11; 10-2009; 1699-1707
dc.identifier.issn
1754-2189
dc.identifier.uri
http://hdl.handle.net/11336/104062
dc.description.abstract
The sunflower belongs to the Compositae family and is an economically important crop because of the quality of its oil. Unfortunately, molecular analyses are limited due to the lack of genomic information, mutant libraries and efficient and rapid transformation protocols. In a wide variety of species, Agrobacterium-mediated transient transformation is a useful tool that can provide valuable insight into many biological processes. However, this technology has not been routinely applied to the sunflower because of difficulties with infiltration. Here, we present an optimized protocol for Agrobacterium-mediated transient transformation of leaf discs. Using this procedure, we were able to quickly overexpress or silence a given gene, enabling us to study several biochemical processes and characterize sunflower regulatory sequences. One of the major advantages of this approach is that in only 1 work-week it is possible to acquire considerable molecular information while avoiding the use of controversial heterologous systems. Transforming heterologous species is frequently unacceptable, as the conservation of molecular events in many cases is not well documented.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
plant transformation
dc.subject
sunflower
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Transient transformation of sunflower leaf discs via an Agrobacterium-mediated method: applications for gene expression and silencing studies
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-28T13:08:22Z
dc.journal.volume
4
dc.journal.number
11
dc.journal.pagination
1699-1707
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.journal.title
Nature Protocols
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/nprot/journal/v4/n11/full/nprot.2009.178.html
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/nprot.2009.178
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