Artículo

Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA)

Bustamante, Ana VictoriaIcon ; Lucchesi, Paula Maria AlejandraIcon ; Parma, Alberto ErnestoIcon
Fecha de publicación: 12/2009
Editorial: Sociedade Brasileira de Microbiologia
Revista: Brazilian Journal of Microbiology
ISSN: 1517-8382
e-ISSN: 1678-4405
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

The aim of this work was to adapt described MLVA protocols to the molecular typing and characterization of VTEC O157:H7 isolates from Argentina. Nine VNTR loci were amplified by PCR showing diversity values from 0.49 to 0.73. Nine MLVA profiles were observed and the cluster analysis indicated both unrelated and closely related VTEC O157:H7 strains. In spite of the limited number of isolates studied, the panel of VNTR used made it possible to perform a first approach of the high genetic diversity of native strains of O157:H7 by MLVA.
Palabras clave: MLVA , VTEC O157:H7 , MOLECULAR CHARACTERIZATION , TYPING
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial 2.5 Unported (CC BY-NC 2.5)
Identificadores
URI: http://hdl.handle.net/11336/104026
DOI: https://doi.org/10.1590/S1517-83822009000400026
URL: http://ref.scielo.org/fwp84c
Colecciones
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Citación
Bustamante, Ana Victoria; Lucchesi, Paula Maria Alejandra; Parma, Alberto Ernesto; Molecular characterization of Verocytotoxigenic Escherichia coli O157:H7 isolates from Argentina by Multiple-Loci VNTR Analysis (MLVA); Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 40; 4; 12-2009; 927-932
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