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dc.contributor.author
Imanishi, Leandro Ezequiel  
dc.contributor.author
Perrine Walker, Francine  
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Ndour, Adama  
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Vayssières, Alice  
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Conejero, Geneviève  
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Lucas, Mikael  
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Champion, Anthony  
dc.contributor.author
Laplaze, Laurent  
dc.contributor.author
Wall, Luis Gabriel  
dc.contributor.author
Svistoonoff Sanchez, Sergio  
dc.date.available
2020-04-29T13:52:35Z  
dc.date.issued
2014-08  
dc.identifier.citation
Imanishi, Leandro Ezequiel; Perrine Walker, Francine; Ndour, Adama; Vayssières, Alice; Conejero, Geneviève; et al.; Role of auxin during intercellular infection of Discaria trinervis by Frankia; Frontiers in Plant Science; Frontiers in Plant Science; 5; 399; 8-2014; 1-9  
dc.identifier.issn
1664-462X  
dc.identifier.uri
http://hdl.handle.net/11336/103884  
dc.description.abstract
Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Frontiers in Plant Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
AUXIN  
dc.subject
ACTINORRHIZA  
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SYSTEMS BIOLOGY  
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ENDOSYMBIOSIS  
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MODEL  
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AUX1  
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PIN  
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NITROGEN FIXATION  
dc.subject.classification
Ciencias de las Plantas, Botánica  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Role of auxin during intercellular infection of Discaria trinervis by Frankia  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-04-28T14:18:25Z  
dc.journal.volume
5  
dc.journal.number
399  
dc.journal.pagination
1-9  
dc.journal.pais
Suiza  
dc.journal.ciudad
Lausana  
dc.description.fil
Fil: Imanishi, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Perrine Walker, Francine. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia  
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Fil: Ndour, Adama. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal  
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Fil: Vayssières, Alice. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia  
dc.description.fil
Fil: Conejero, Geneviève. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia  
dc.description.fil
Fil: Lucas, Mikael. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia  
dc.description.fil
Fil: Champion, Anthony. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia  
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Fil: Laplaze, Laurent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal  
dc.description.fil
Fil: Wall, Luis Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina  
dc.description.fil
Fil: Svistoonoff Sanchez, Sergio. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia  
dc.journal.title
Frontiers in Plant Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fpls.2014.00399  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2014.00399/full