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dc.contributor.author
Imanishi, Leandro Ezequiel
dc.contributor.author
Perrine Walker, Francine
dc.contributor.author
Ndour, Adama
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Vayssières, Alice
dc.contributor.author
Conejero, Geneviève
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Lucas, Mikael
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Champion, Anthony
dc.contributor.author
Laplaze, Laurent
dc.contributor.author
Wall, Luis Gabriel
dc.contributor.author
Svistoonoff Sanchez, Sergio
dc.date.available
2020-04-29T13:52:35Z
dc.date.issued
2014-08
dc.identifier.citation
Imanishi, Leandro Ezequiel; Perrine Walker, Francine; Ndour, Adama; Vayssières, Alice; Conejero, Geneviève; et al.; Role of auxin during intercellular infection of Discaria trinervis by Frankia; Frontiers in Plant Science; Frontiers in Plant Science; 5; 399; 8-2014; 1-9
dc.identifier.issn
1664-462X
dc.identifier.uri
http://hdl.handle.net/11336/103884
dc.description.abstract
Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers in Plant Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
AUXIN
dc.subject
ACTINORRHIZA
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SYSTEMS BIOLOGY
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ENDOSYMBIOSIS
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MODEL
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AUX1
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PIN
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NITROGEN FIXATION
dc.subject.classification
Ciencias de las Plantas, Botánica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Role of auxin during intercellular infection of Discaria trinervis by Frankia
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-28T14:18:25Z
dc.journal.volume
5
dc.journal.number
399
dc.journal.pagination
1-9
dc.journal.pais
Suiza
dc.journal.ciudad
Lausana
dc.description.fil
Fil: Imanishi, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
dc.description.fil
Fil: Perrine Walker, Francine. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
dc.description.fil
Fil: Ndour, Adama. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal
dc.description.fil
Fil: Vayssières, Alice. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
dc.description.fil
Fil: Conejero, Geneviève. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Lucas, Mikael. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
dc.description.fil
Fil: Champion, Anthony. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
dc.description.fil
Fil: Laplaze, Laurent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal
dc.description.fil
Fil: Wall, Luis Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
dc.description.fil
Fil: Svistoonoff Sanchez, Sergio. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
dc.journal.title
Frontiers in Plant Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fpls.2014.00399
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2014.00399/full
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