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Artículo

Codon optimization of bacterial luciferase (lux) for expression in mammalian cells

Patterson, Stacey S.; Dionisi, Hebe MonicaIcon ; Gupta, Rakesh K.; Sayler, Gary S.
Fecha de publicación: 03/2005
Editorial: Springer Heidelberg
Revista: Journal of Industrial Microbiology & Biotechnology
ISSN: 1367-5435
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Expression of the bacterial luciferase (lux) system in mammalian cells would culminate in a new generation of bioreporters for in vivo monitoring and diagnostics technology. Past efforts to express bacterial luciferase in mammalian cells have resulted in only modest gains due in part to low overall expression of the bacterial genes. To optimize expression, we have designed and synthesized codon-optimized versions of the luxA and luxB genes from Photorhabdus luminsecens. To evaluate these genes in vivo, stable HEK293 cell lines were created harboring wild type luxA and luxB (WTA/WTB), codon-optimized luxA and wild type luxB (COA/WTB), and codon-optimized versions of both luxA and luxB genes (COA/COB). Although mRNA levels within these clones remained approximately equal, LuxA protein levels increased significantly after codon optimization. On average, bioluminescence levels were increased by more than six-fold [5×105 vs 2.9×106 relative light units (RLU)/mg total protein] with the codon-optimized luxA and wild type luxB. Bioluminescence was further enhanced upon expression of both optimized genes (2.7×107 RLU/mg total protein). These results show promise toward the potential development of an autonomous light generating lux reporter system in mammalian cells.
Palabras clave: BIOLUMINESCENCE , BIOREPORTER PROTEIN , EUKARYOTIC EXPRESSION
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/103821
DOI: http://dx.doi.org/10.1007/s10295-005-0211-8
URL: https://academic.oup.com/jimb/article/32/3/115/5992712
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Articulos(CCT-CENPAT)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CENPAT
Citación
Patterson, Stacey S.; Dionisi, Hebe Monica; Gupta, Rakesh K.; Sayler, Gary S.; Codon optimization of bacterial luciferase (lux) for expression in mammalian cells; Springer Heidelberg; Journal of Industrial Microbiology & Biotechnology; 32; 3; 3-2005; 115-123
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