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dc.contributor.author
Mottier, Maria de Lourdes
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dc.contributor.author
Alvarez, Luis Ignacio
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dc.contributor.author
Fairweather, Ian
dc.contributor.author
Lanusse, Carlos Edmundo
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dc.date.available
2020-04-28T18:13:04Z
dc.date.issued
2006-12
dc.identifier.citation
Mottier, Maria de Lourdes; Alvarez, Luis Ignacio; Fairweather, Ian; Lanusse, Carlos Edmundo; Resistance induced changes on triclabendazole transport in Fasciola hepatica: ivermectin reversal effect; American Society of Parasitologists; Journal of Parasitology; 92; 6; 12-2006; 1355-1360
dc.identifier.issn
0022-3395
dc.identifier.uri
http://hdl.handle.net/11336/103799
dc.description.abstract
Triclabendazole (TCBZ) and albendazole (ABZ) are flukicidal benzimidazole compounds extensively used in veterinary medicine. Although TCBZ has excellent activity against mature and immature stages of the liver fluke, Fasciola hepatica, ABZ action is restricted to flukes older than 12 wk. The intensive use of TCBZ has resulted in the development of resistance. To gain insight into the mechanisms of resistance to TCBZ, the ex vivo diffusion of TCBZ, TCBZ sulfoxide (TCBZSO, the active metabolite of TCBZ), and ABZ into TCBZ-susceptible and -resistant adult flukes was compared. TCBZ-susceptible (Cullompton) and -resistant (Sligo) flukes were incubated in Krebs-Ringer Tris buffer with either TCBZ, TCBZSO, or ABZ (5 nmol/ ml) for 90 min. Drug/metabolite concentrations were quantified by high-performance liquid chromatography. All the assayed molecules penetrated through the tegument of both susceptible and resistant flukes. However, significantly lower concentrations of TCBZ and TCBZSO were recovered within the TCBZ-resistant flukes. In contrast, ABZ entrance into the susceptible and resistant flukes was equivalent. The influx/efflux balance for TCBZ, TCBZSO, and ABZ in susceptible and resistant flukes in the presence or absence of a substrate (ivermectin) of the drug transporter P-glycoprotein was assessed. The ivermectin-induced modulation of P-glycoprotein activity decreased TCBZ efflux from the resistant flukes. Higher concentrations of TCBZ and TCBZSO were recovered from the resistant liver flukes in the presence of ivermectin. Thus, an altered influx/efflux mechanism may account for the development of resistance to TCBZ in F. hepatica.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Parasitologists
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
TRICLABENDAZOLE
dc.subject
RESISTANCE LIVER FLUKES
dc.subject
TRANSPORT MODULATION
dc.subject
IVERMECTIN
dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
Ciencias Veterinarias
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Resistance induced changes on triclabendazole transport in Fasciola hepatica: ivermectin reversal effect
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-17T14:42:36Z
dc.identifier.eissn
1937-2345
dc.journal.volume
92
dc.journal.number
6
dc.journal.pagination
1355-1360
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Mottier, Maria de Lourdes. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Alvarez, Luis Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Fairweather, Ian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. The Queens University of Belfast; Irlanda
dc.description.fil
Fil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal of Parasitology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1645/GE-922R.1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/journal-of-parasitology/volume-92/issue-6/GE-922R.1/RESISTANCE-INDUCED-CHANGES-IN-TRICLABENDAZOLE-TRANSPORT-IN-FASCIOLA-HEPATICA/10.1645/GE-922R.1.short
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