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dc.contributor.author
Murgida, Daniel Horacio
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dc.contributor.author
von Stetten, David
dc.contributor.author
Hildebrandt, Peter
dc.contributor.author
Schwinté, Pascale
dc.contributor.author
Siebert, Friedrich
dc.contributor.author
Sharda, Shivani
dc.contributor.author
Gärtner, Wolfgang
dc.contributor.author
Mroginski, Maria Andrea
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dc.date.available
2020-04-23T15:36:49Z
dc.date.issued
2007-10
dc.identifier.citation
Murgida, Daniel Horacio; von Stetten, David; Hildebrandt, Peter; Schwinté, Pascale; Siebert, Friedrich; et al.; The Chromophore Structures of the Pr States in Plant and Bacterial Phytochromes; Cell Press; Biophysical Journal; 93; 7; 10-2007; 2410-2417
dc.identifier.issn
0006-3495
dc.identifier.uri
http://hdl.handle.net/11336/103445
dc.description.abstract
The resonance Raman spectra of the Pr state of the N-terminal 65-kDa fragment of plant phytochrome phyA have been measured and analyzed in terms of the configuration and conformation of the tetrapyrroles methine bridges. Spectra were obtained from phyA adducts reconstituted with the natural chromophore phytochromobilin as well as phycocyanobilin and its isotopomers labeled at the terminal methine bridges through C-13/C-12 and D/H substitution. Upon comparing the resonance Raman spectra of the various phyA adducts, it was possible to identify the bands that originate from normal modes dominated by the stretching coordinates of the terminal methine bridges A-B and C-D. Quantum chemical calculations of the isolated tetrapyrroles reveal that these modes are sensitive indicators for the methine bridge configuration and conformation. For all phyA adducts, the experimental spectra of Pr including this marker band region are well reproduced by the calculated spectra obtained for the ZZZasa configuration. In contrast, there are substantial discrepancies between the experimental spectra and the spectra calculated for the ZZZssa configuration, which has been previously shown to be the chromophore geometry in the Pr state of the bacterial, biliverdin-binding phytochrome from Deinococcus radiodurans (Wagner, J. R., J. S. Brunzelle, K. T. Forest, R. D. Vierstra. 2005. Nature. 438: 325-331). The results of this work, therefore, suggest that plant and bacterial (biliverdin-binding) phytochromes exhibit different structures in the parent state although the mechanism of the photoinduced reaction cycle may be quite similar.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cell Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Phytochrome
dc.subject
Photoreceptors
dc.subject
FT-Raman
dc.subject
DFT simulations
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The Chromophore Structures of the Pr States in Plant and Bacterial Phytochromes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-22T15:40:54Z
dc.journal.volume
93
dc.journal.number
7
dc.journal.pagination
2410-2417
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
United States
dc.description.fil
Fil: Murgida, Daniel Horacio. Technische Universitat Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
dc.description.fil
Fil: von Stetten, David. Technische Universitat Berlin; Alemania
dc.description.fil
Fil: Hildebrandt, Peter. Technische Universitat Berlin; Alemania
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Fil: Schwinté, Pascale. Universidad de Friburgo; Alemania
dc.description.fil
Fil: Siebert, Friedrich. Universidad de Friburgo; Alemania
dc.description.fil
Fil: Sharda, Shivani. Max-Planck-Institut fur Bioanorganische Chemie; Alemania
dc.description.fil
Fil: Gärtner, Wolfgang. Technische Universitat Berlin; Alemania. Max-Planck-Institut fur Bioanorganische Chemie; Alemania
dc.description.fil
Fil: Mroginski, Maria Andrea. Max-Planck-Institut fur Bioanorganische Chemie; Alemania
dc.journal.title
Biophysical Journal
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006349507714960
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1529/biophysj.107.108092
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