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dc.contributor.author
Pocognoni, Cristián Adrián
dc.contributor.author
de Blas, Gerardo Andrés
dc.contributor.author
Heuck, Alejandro P.
dc.contributor.author
Belmonte, Silvia Alejandra
dc.contributor.author
Mayorga, Luis Segundo
dc.date.available
2016-12-27T19:51:10Z
dc.date.issued
2013-01
dc.identifier.citation
Pocognoni, Cristián Adrián; de Blas, Gerardo Andrés; Heuck, Alejandro P.; Belmonte, Silvia Alejandra; Mayorga, Luis Segundo; Perfringolysin O as a useful tool to study human sperm physiology; Elsevier; Fertility And Sterility; 99; 1; 1-2013; 99-106.e2
dc.identifier.issn
0015-0282
dc.identifier.uri
http://hdl.handle.net/11336/10332
dc.description.abstract
Objective: To evaluate perfringolysin O, a cholesterol-dependent pore-forming cytolysin, as a tool to study several aspects of human sperm physiology. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Human semen samples with normal parameters obtained from healthy donors. Intervention(s): Interaction of recombinant perfringolysin O with human spermatozoa. Main Outcome Measure(s): Assessment of perfringolysin O binding to spermatozoa, tests for acrosome and plasma membrane integrity, and acrosomal exocytosis assays. Result(s): Perfringolysin O associated with human spermatozoa at 4 C. The binding was sensitive to changes in cholesterol concentrations and distribution occurring in the plasma membrane of these cells during capacitation. When perfringolysin O–treated sperm were incubated at 37 C, the plasma membrane became permeable, whereas the acrosome membrane remained intact. Permeabilized spermatozoa were able to respond to exocytic stimuli. The process was inhibited by proteins that interfere with membrane fusion, indicating that large molecules, including antibodies, were able to permeate into the spermatozoa. Conclusion(s): PFO is a useful probe to assess changes in the amount and distribution of the active sterol fraction present in the sperm plasma membrane. The toxin can be used for the efficient and selective permeabilization of this membrane, rendering a flexible experimental model suitable for studying molecular processes occurring in the sperm cytoplasm. (Fertil Steril 2013;99:99–106. 2013 by American Society for Reproductive Medicine).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Perfringgolysin O
dc.subject
Acrosome Reaction
dc.subject
Human Sperm
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Perfringolysin O as a useful tool to study human sperm physiology
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-09-19T18:46:42Z
dc.journal.volume
99
dc.journal.number
1
dc.journal.pagination
99-106.e2
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Pocognoni, Cristián Adrián. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: de Blas, Gerardo Andrés. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: Heuck, Alejandro P.. University of Massachusett. Department of
Biochemistry and Molecular Biology; Estados Unidos
dc.description.fil
Fil: Belmonte, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.description.fil
Fil: Mayorga, Luis Segundo. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina
dc.journal.title
Fertility And Sterility
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0015028212021401
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.fertnstert.2012.08.052
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