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dc.contributor.author
Salazar, Nuria
dc.contributor.author
Binetti, Ana Griselda
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dc.contributor.author
Gueimonde, Miguel
dc.contributor.author
Alonso, Ana
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Garrido, Pablo
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González del Rey, Carmen
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González, Celestino
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Ruas-Madiedo, Patricia
dc.contributor.author
de los Reyes-Gavilán, Clara G.
dc.date.available
2020-04-21T17:57:52Z
dc.date.issued
2011-01
dc.identifier.citation
Salazar, Nuria; Binetti, Ana Griselda; Gueimonde, Miguel; Alonso, Ana; Garrido, Pablo; et al.; Safety and intestinal microbiota modulation by the exopolysaccharide-producing strains Bifidobacterium animalis IPLA R1 and Bifidobacterium longum IPLA E44 orally administered to Wistar rats; Elsevier Science; International Journal of Food Microbiology; 144; 3; 1-2011; 342-351
dc.identifier.issn
0168-1605
dc.identifier.uri
http://hdl.handle.net/11336/103205
dc.description.abstract
Bifidobacterium animalis subsp. lactis IPLA R1 and Bifidobacterium longum IPLA E44 strains were tested in vivo for their safety and ability to modulate the intestinal microbiota. Chemically simulated gastrointestinal digestion showed a considerably lower survival of E44 than R1 strain, the first microorganism being also more sensitive to the refrigerated storage in 10%-skimmed milk at 4ºC. Male Wistar rats 12 weeks-old were distributed into three groups, eight rats each. Two groups were daily administered 108 to 109 cfu of the corresponding strain suspended in 10%-skimmed milk during 24 days whereas rats in the placebo group received skimmed milk without microorganisms added. The microbiota and short chain fatty acids (SCFA) were monitored daily in faeces during treatment and in caecum-content at the end of the assay. Quantitative real-time PCR (qPCR) showed that faecal and caecal Bifidobacterium levels were higher in bifidobacteria-fed than in the placebo rats at the end of the intervention whereas total anaerobic-plate counts did not show significant differences. Quantification of B. animalis and B. longum by q-PCR evidenced that, independently on the microorganism administered, treatment with bifidobacteria resulted in higher levels of B. animalis in the caecum. PCR-DGGE analysis of microbial populations revealed a higher diversity of bands in caecum-content of rats fed B. animalis IPLA R1 than in the placebo and rats fed B. longum IPLA E44. Remarkably, although no variations in the proportion of acetate, propionate and butyrate were found, at the end of the assay total SCFA concentration in faeces of rats fed bifidobacteria were significantly higher and those in caecum-content significantly lower than that of the placebo group, suggesting a displacement of the SCFA production to parts in the intestine more distal that caecum in rats receiving bifidobacteria. Harmful glycosidic activities were absent or at low levels in the strains R1 and E44. Both strains were sensitive to most antibiotics although, similarly to some other bifidobacteria strains, they displayed a moderate resistance against tetracycline which in the case of R1correlated with the presence of tet (W) gene. The general parameters indicating well-being status as well as translocation to different organs and histological examination of the gut tissues revealed no changes induced by the administration of bifidobacteria. Therefore, the oral administration of B. animalis IPLA R1 and B. longum E44 can be considered safe, these microorganisms having the ability to modulate the intestinal microbiota of rats by influencing SCFA and the bifidobacterial population levels.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
probiotic
dc.subject
bifidobacteria
dc.subject
exopolysacharide
dc.subject.classification
Alimentos y Bebidas
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dc.subject.classification
Otras Ingenierías y Tecnologías
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Safety and intestinal microbiota modulation by the exopolysaccharide-producing strains Bifidobacterium animalis IPLA R1 and Bifidobacterium longum IPLA E44 orally administered to Wistar rats
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-21T15:27:12Z
dc.journal.volume
144
dc.journal.number
3
dc.journal.pagination
342-351
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Salazar, Nuria. Instituto de Productos Lácteos de Asturias; España
dc.description.fil
Fil: Binetti, Ana Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
dc.description.fil
Fil: Gueimonde, Miguel. Instituto de Productos Lácteos de Asturias; España
dc.description.fil
Fil: Alonso, Ana. Universidad de Oviedo; España
dc.description.fil
Fil: Garrido, Pablo. Universidad de Oviedo; España
dc.description.fil
Fil: González del Rey, Carmen. Agustin Hospital; España
dc.description.fil
Fil: González, Celestino. Universidad de Oviedo; España
dc.description.fil
Fil: Ruas-Madiedo, Patricia. Instituto de Productos Lácteos de Asturias; España
dc.description.fil
Fil: de los Reyes-Gavilán, Clara G.. Instituto de Productos Lácteos de Asturias; España
dc.journal.title
International Journal of Food Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijfoodmicro.2010.10.016
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