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dc.contributor.author
Testa, Ilaria
dc.contributor.author
Schönle, Andreas
dc.contributor.author
V. Middendorff, Claas
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Geisler, Claudia
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Medda, Rebecca
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Wurm, Christian A.
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Stiel, Andre C.
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Jakobs, Stefan
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Bossi, Mariano Luis
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Eggeling, Christian
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Hell, Stefan W.
dc.contributor.author
Egner, Alexander
dc.date.available
2020-04-21T17:55:49Z
dc.date.issued
2008-12
dc.identifier.citation
Testa, Ilaria; Schönle, Andreas; V. Middendorff, Claas; Geisler, Claudia; Medda, Rebecca; et al.; Nanoscale separation of molecular species based on their rotational mobility; Optical Society of America; Optics Express; 16; 25; 12-2008; 21093-21104
dc.identifier.issn
1094-4087
dc.identifier.uri
http://hdl.handle.net/11336/103202
dc.description.abstract
We combine far-field fluorescence nanoscopy through serializedrecording of switchable emitters with polarization-sensitive fluorescencedetection. In addition to imaging with nanoscale spatial resolution, thistechnique allows determination of the fluorescence anisotropy of eachdetected dipole emitter and thus an estimate of its rotational mobility. Subpopulationsof fluorescent markers can thus be separated based on theirinteraction with the sample. We applied this new functional nanoscopy toimaging of living mammalian cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Optical Society of America
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Fluorescence
dc.subject
Microscopy
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biological imaging
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Físico-Química, Ciencia de los Polímeros, Electroquímica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Nanoscale separation of molecular species based on their rotational mobility
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-17T14:42:17Z
dc.journal.volume
16
dc.journal.number
25
dc.journal.pagination
21093-21104
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Testa, Ilaria. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Schönle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: V. Middendorff, Claas. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Geisler, Claudia. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Stiel, Andre C.. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Bossi, Mariano Luis. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
dc.description.fil
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania
dc.journal.title
Optics Express
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/oe/abstract.cfm?uri=oe-16-25-21093
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1364/OE.16.021093
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