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dc.contributor.author
Fölling, Jonas
dc.contributor.author
Bossi, Mariano Luis
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Bock, Hannes
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Medda, Rebecca
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Wurm, Christian A
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Hein, Birka
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Jakobs, Stefan
dc.contributor.author
Eggeling, Christian
dc.contributor.author
Hell, Stefan W
dc.date.available
2020-04-20T15:32:00Z
dc.date.issued
2008-09
dc.identifier.citation
Fölling, Jonas; Bossi, Mariano Luis; Bock, Hannes; Medda, Rebecca; Wurm, Christian A; et al.; Fluorescence nanoscopy by ground-state depletion and single-molecule return; Nature Publishing Group; Nature Methods; 5; 11; 9-2008; 943-945
dc.identifier.issn
1548-7091
dc.identifier.uri
http://hdl.handle.net/11336/103015
dc.description.abstract
We introduce far-field fluorescence nanoscopy with ordinaryfluorophores based on switching the majority of them to ametastable dark state, such as the triplet, and calculating theposition of those left or those that spontaneously returned to theground state. Continuous widefield illumination by a single laserand a continuously operating camera yielded dual-color imagesof rhodamine- and fluorescent protein–labeled (living) samples,proving a simple yet powerful super-resolution approach.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
fluorescence nanoscopy
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single molecules
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super-resolution
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Fluorescence nanoscopy by ground-state depletion and single-molecule return
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-17T14:42:25Z
dc.journal.volume
5
dc.journal.number
11
dc.journal.pagination
943-945
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Fölling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Bock, Hannes. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania
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Fil: Wurm, Christian A. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Hein, Birka. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
dc.description.fil
Fil: Hell, Stefan W. Max Planck Institute for Biophysical Chemistry; Alemania
dc.journal.title
Nature Methods
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/nmeth.1257
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/nmeth.1257
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