Mostrar el registro sencillo del ítem

dc.contributor.author
Saucedo, Lucia
dc.contributor.author
Buffa, Gabriela Natalia
dc.contributor.author
Rosso, Marina
dc.contributor.author
Guillardoy, Tomás
dc.contributor.author
Gongora, Adrian Daniel
dc.contributor.author
Munuce, María J.
dc.contributor.author
Vazquez, Monica Hebe
dc.contributor.author
Marin Briggiler, Clara Isabel
dc.date.available
2016-12-27T16:06:01Z
dc.date.issued
2015-05-13
dc.identifier.citation
Saucedo, Lucia; Buffa, Gabriela Natalia; Rosso, Marina; Guillardoy, Tomás; Gongora, Adrian Daniel; et al.; Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation; Public Library Of Science; Plos One; 10; 5; 13-5-2015; e0127297-e0127297
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/10300
dc.description.abstract
Fibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Sperm
dc.subject
Fibroblast Growth Factor
dc.subject
Fgfr
dc.subject
Fgf
dc.subject.classification
Fisiología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-10-04T19:25:25Z
dc.identifier.eissn
1932-6203
dc.journal.volume
10
dc.journal.number
5
dc.journal.pagination
e0127297-e0127297
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Buffa, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Guillardoy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Gongora, Adrian Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Munuce, María J.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Sanatorio Británico de Rosario. Reprolab; Argentina
dc.description.fil
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127297
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0127297
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430232/


Archivos asociados

 

Comunidades y colecciones

  • Articulos(IBYME) [1100]
    Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)

Mostrar el registro sencillo del ítem