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dc.contributor.author
Saucedo, Lucia  
dc.contributor.author
Buffa, Gabriela Natalia  
dc.contributor.author
Rosso, Marina  
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Guillardoy, Tomás  
dc.contributor.author
Gongora, Adrian Daniel  
dc.contributor.author
Munuce, María J.  
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Vazquez, Monica Hebe  
dc.contributor.author
Marin Briggiler, Clara Isabel  
dc.date.available
2016-12-27T16:06:01Z  
dc.date.issued
2015-05-13  
dc.identifier.citation
Saucedo, Lucia; Buffa, Gabriela Natalia; Rosso, Marina; Guillardoy, Tomás; Gongora, Adrian Daniel; et al.; Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation; Public Library Of Science; Plos One; 10; 5; 13-5-2015; e0127297-e0127297  
dc.identifier.issn
1932-6203  
dc.identifier.uri
http://hdl.handle.net/11336/10300  
dc.description.abstract
Fibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Public Library Of Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
Sperm  
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Fibroblast Growth Factor  
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Fgfr  
dc.subject
Fgf  
dc.subject.classification
Fisiología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Fibroblast Growth Factor Receptors (FGFRs) in human male germ cells: evidence for their expression, functionality and involvement in sperm motility regulation  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-10-04T19:25:25Z  
dc.identifier.eissn
1932-6203  
dc.journal.volume
10  
dc.journal.number
5  
dc.journal.pagination
e0127297-e0127297  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
San Francisco  
dc.description.fil
Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina  
dc.description.fil
Fil: Buffa, Gabriela Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina  
dc.description.fil
Fil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina  
dc.description.fil
Fil: Guillardoy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina  
dc.description.fil
Fil: Gongora, Adrian Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina  
dc.description.fil
Fil: Munuce, María J.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Sanatorio Británico de Rosario. Reprolab; Argentina  
dc.description.fil
Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina  
dc.description.fil
Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina  
dc.journal.title
Plos One  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127297  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0127297  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430232/