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dc.contributor.author
Corigliano, Mariana Georgina
dc.contributor.author
de Guzmán, Ana María Stefanini
dc.contributor.author
Stagnitta, Patricia Virginia
dc.date.available
2020-04-14T20:37:52Z
dc.date.issued
2011-03
dc.identifier.citation
Corigliano, Mariana Georgina; de Guzmán, Ana María Stefanini; Stagnitta, Patricia Virginia; Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina; National Institute of Public Health; Central European Journal of Public Health; 19; 1; 3-2011; 46-53
dc.identifier.issn
1803-1048
dc.identifier.uri
http://hdl.handle.net/11336/102555
dc.description.abstract
Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production.The aim of this work was: i) to assess the sanitary-hygienic quality of dehydrated soups (100 samples) consumed in San Luis – Argentina; ii) to verify the presence of C. perfringens in these food products using the "Most Probable Number" method (MPN) and plate-counting methods; iii) to characterise enterotoxigenicity in strain isolates by RPLA; iv) to determine the chromosomal or plasmidic location of the cpe gene in enterotoxigenic strains previously isolated from food in our lab, using PCR; v) to correlate chromosomal cpe and spore heat-resistance; vi) to compare protease activity in cpe+ and cpe– strains; and vii) to compare azoreductase activity in cpe+ and cpe– strains. Twenty-six isolates had a count a 3–43 bacteria g-1 count using MPN; 7.7% exceeded the Argentine Food Code (CAA) limit. All isolates showed protease activity: enterotoxigenic isolates had higher protease activity than non-enterotoxigenic isolates. All isolates showed azoreductase activity: enterotoxigenic isolates had higher activity and shorter reducing times. Enterotoxigenic isolates showed chromosomal location for the gene responsible for the enterotoxin.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Institute of Public Health
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ENTEROTOXIN
dc.subject
PROTEASE
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AZOREDUCTASE
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food
dc.subject
clostridium perfringens
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-08T18:15:20Z
dc.journal.volume
19
dc.journal.number
1
dc.journal.pagination
46-53
dc.journal.pais
República Checa
dc.description.fil
Fil: Corigliano, Mariana Georgina. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: de Guzmán, Ana María Stefanini. Universidad Nacional de San Luis; Argentina
dc.description.fil
Fil: Stagnitta, Patricia Virginia. Universidad Nacional de San Luis; Argentina
dc.journal.title
Central European Journal of Public Health
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www1.szu.cz/svi/cejph/show.php?kat=archiv/2011-1-09
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.21101/cejph.a3597
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