Artículo
Functional Capacity of Shiga-Toxin Promoter Sequences in Eukaryotic Cells
Bentancor, Leticia Verónica
; Bilen, Marcos Fabian
; Mejias, María Pilar
; Fernández Brando, Romina Jimena
; Panek, Cecilia Analía
; Ramos, Maria Victoria
; Fernández, Gabriela Cristina
; Isturiz, Martín Amadeo
; Ghiringhelli, Pablo Daniel
; Palermo, Marina Sandra
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Fecha de publicación:
02/2013
Editorial:
Public Library of Science
Revista:
Plos One
ISSN:
1932-6203
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Shiga toxins (Stx) are the main virulence factors in enterohemorrhagic Escherichia coli (EHEC) infections, causing diarrhea and hemolytic uremic syndrome (HUS). The genes encoding for Shiga toxin-2 (Stx2) are located in a bacteriophage. The toxin is formed by a single A subunit and five B subunits, each of which has its own promoter sequence. We have previously reported the expression of the B subunit within the eukaryotic environment, probably driven by their own promoter. The aim of this work was to evaluate the ability of the eukaryotic machinery to recognize stx2 sequences as eukaryotic-like promoters. Vero cells were transfected with a plasmid encoding Stx2 under its own promoter. The cytotoxic effect on these cells was similar to that observed upon incubation with purified Stx2. In addition, we showed that Stx2 expression in Stx2-insensitive BHK eukaryotic cells induced drastic morphological and cytoskeletal changes. In order to directly evaluate the capacity of the wild promoter sequences of the A and B subunits to drive protein expression in mammalian cells, GFP was cloned under eukaryotic-like putative promoter sequences. GFP expression was observed in 293T cells transfected with these constructions. These results show a novel and alternative way to synthesize Stx2 that could contribute to the global understanding of EHEC infections with immediate impact on the development of treatments or vaccines against HUS
Palabras clave:
BACTERIOPHAGE
,
SHIGA TOXIN
,
HEMOLYTIC UREMIC SYNDROME (HUS)
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Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Articulos de INST.DE MEDICINA EXPERIMENTAL
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Bentancor, Leticia Verónica; Bilen, Marcos Fabian; Mejias, María Pilar; Fernández Brando, Romina Jimena; Panek, Cecilia Analía; et al.; Functional Capacity of Shiga-Toxin Promoter Sequences in Eukaryotic Cells; Public Library of Science; Plos One; 8; 2; 2-2013; 1-14
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