Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus lineatus (Pisces, Curimatidae)

Toxicants on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using alkaline comet assay applied on erythrocytes after in vivo exposure of Prochilodus lineatus to different concentrations of Cypermethrin (0.300, 0.150, 0.075 and 0.000 µg/L) as a probable chemical mutagen. The results revealed a significantly higher level of DNA damage at all concentrations of Cypermethrin tested compared to control and background level (p<0.05). We have standardized the technique for one of the most common native fish species that will be useful for biomonitoring genotoxicity in polluted waters of the region.

Palabras clave: Prochilodus lineatus , COMET ASSAY , GENOTOXIC EFFECTS , CYPERMETHRIN

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

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URI: http://hdl.handle.net/11336/102237

DOI: http://dx.doi.org/10.1007/s00128-009-9771-z

URL: https://link.springer.com/article/10.1007/s00128-009-9771-z

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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE

Citación

Simoniello, Maria Fernanda; Gigena, F.; Poletta, Gisela Laura; Loteste, Alicia Elena; Kleinsorge, E.; et al.; Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus lineatus (Pisces, Curimatidae); Springer; Bulletin of Environmental Contamination and Toxicology; 83; 2; 5-2009; 155-158

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