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dc.contributor.author
Paravani, Enrique Valentin
dc.contributor.author
Simoniello, Maria Fernanda
dc.contributor.author
Poletta, Gisela Laura
dc.contributor.author
Zolessi, F.R.
dc.contributor.author
Casco, Victor Hugo
dc.date.available
2020-04-07T14:07:43Z
dc.date.issued
2018
dc.identifier.citation
Paravani, Enrique Valentin; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Zolessi, F.R.; Casco, Victor Hugo; Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish; Elsevier Science; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 826; 2018; 25-32
dc.identifier.issn
1383-5718
dc.identifier.uri
http://hdl.handle.net/11336/102155
dc.description.abstract
Cypermethrin (CM), widely used for control of indoor and field pests, is one of the most common contaminantsin freshwater aquatic systems. We evaluated CM genotoxicity and the activities of superoxide dismutase (SOD)and catalase (CAT) in retinal cells of adult zebrafish. Histological and immunofluorescence techniques show thepresence of apoptotic cells in the zebrafish retina after 9 d of treatment with 0.6 μg/L CM. Histone γ-H2AX, amarker of DNA damage, was detected in both outer and inner nuclear layers; caspase-3, an apoptotic marker,was detected in the outer nuclear layer. In the comet assay, the cells were sensitive to hydrogen peroxideinducedDNA damage, showing a dose-dependent response. We observed a positive comet assay response to CMthat was dose- and time-dependent. Following exposure to CM, SOD and CAT enzyme activities, and sod and catmRNA levels, increased. These results indicate that CM causes DNA damage and oxidative stress and can induceapoptosis in retinal cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DNA damage
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Apoptosis
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Apoptosis
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Cypermethrin: Oxidative stress and genotoxicity in retinal cells of the adult zebrafish
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-02T13:19:33Z
dc.journal.volume
826
dc.journal.pagination
25-32
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos; Argentina
dc.description.fil
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
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Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Zolessi, F.R.. Universidad de la Republica.; Uruguay
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Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos; Argentina
dc.journal.title
Mutation Research. Genetic Toxicology And Environmental Mutagenesis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mrgentox.2017.12.010
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