Artículo
Photochemical formation of a fluorescent thymidine-pterin adduct in DNA
Estébanez, Sandra
; Lorente, Carolina
; Gaspar Tosato, Maira
; Miranda, Miguel A.; Marín, M. Luisa; Lhiaubet-Vallet, Virginie; Thomas, Andrés Héctor
Fecha de publicación:
09/2018
Editorial:
Elsevier
Revista:
Dyes and Pigments
ISSN:
0143-7208
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The photochemistry of the DNA biomacromolecule is an issue of paramount importance as it is part of the etiology of skin cancer development, being ultraviolet sunlight radiation the most relevant environmental carcinogen. Herein, we demonstrate the potential of pterin, an endogenous compound, to form covalent adduct under UVA irradiation with a short thymine oligomer as well as with the whole DNA polymer. Our approach is based on the spectroscopic features of pterin, which allow, by monitoring specific absorption or emission wavelengths, the following-up of the covalent binding. The results are confirmed by HPLC coupled with mass spectrometry, revealing the attachment of one or two pterin units to the homothymine 5-mer oligonucleotide. Altogether the findings point toward the role of pterin as endogenous sensitizer and genotoxic compound.
Palabras clave:
PTERINS
,
THYMINE
,
PHOTOSENSITIZATION
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Articulos(INIFTA)
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Citación
Estébanez, Sandra; Lorente, Carolina; Gaspar Tosato, Maira; Miranda, Miguel A.; Marín, M. Luisa; et al.; Photochemical formation of a fluorescent thymidine-pterin adduct in DNA; Elsevier; Dyes and Pigments; 160; 9-2018; 624-632
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