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dc.contributor.author
Owens, Dawn A.
dc.contributor.author
Butler, Amanda M.
dc.contributor.author
Agüero, Tristán Horacio
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dc.contributor.author
Newman, Karen M.
dc.contributor.author
Van Booven, Derek
dc.contributor.author
King, Mary Lou
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dc.date.available
2020-04-02T18:42:42Z
dc.date.issued
2017-01
dc.identifier.citation
Owens, Dawn A.; Butler, Amanda M.; Agüero, Tristán Horacio; Newman, Karen M.; Van Booven, Derek; et al.; High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration; Company of Biologists; Development; 144; 2; 1-2017; 292-304
dc.identifier.issn
0950-1991
dc.identifier.uri
http://hdl.handle.net/11336/101695
dc.description.abstract
During oogenesis, hundreds of maternal RNAs are selectively localized to the animal or vegetal pole, including determinants of somatic and germline fates. Although microarray analysis has identified localized determinants, it is not comprehensive and is limited to known transcripts. Here, we utilized high-throughput RNA sequencing analysis to comprehensively interrogate animal and vegetal pole RNAs in the fully grown Xenopus laevis oocyte. We identified 411 (198 annotated) and 27 (15 annotated) enriched mRNAs at the vegetal and animal pole, respectively. Ninety were novel mRNAs over 4-fold enriched at the vegetal pole and six were over 10-fold enriched at the animal pole. Unlike mRNAs, microRNAs were not asymmetrically distributed. Whole-mount in situ hybridization confirmed that all 17 selected mRNAs were localized. Biological function and network analysis of vegetally enriched transcripts identified protein-modifying enzymes, receptors, ligands, RNA-binding proteins, transcription factors and co-factors with five defining hubs linking 47 genes in a network. Initial functional studies of maternal vegetally localized mRNAs show that sox7 plays a novel and important role in primordial germ cell (PGC) development and that ephrinB1 (efnb1) is required for proper PGC migration. We propose potential pathways operating at the vegetal pole that highlight where future investigations might be most fruitful.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
XENOPUS
dc.subject
RNA-SEQ
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PGCS
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GENE PATHWAYS
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LOCALIZED RNAS
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SCAFFOLD
dc.subject.classification
Biología del Desarrollo
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-11-25T18:19:19Z
dc.identifier.eissn
1477-9129
dc.journal.volume
144
dc.journal.number
2
dc.journal.pagination
292-304
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Owens, Dawn A.. University of Miami; Estados Unidos
dc.description.fil
Fil: Butler, Amanda M.. University of Miami; Estados Unidos
dc.description.fil
Fil: Agüero, Tristán Horacio. University of Miami; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Newman, Karen M.. University of Miami; Estados Unidos
dc.description.fil
Fil: Van Booven, Derek. University of Miami; Estados Unidos
dc.description.fil
Fil: King, Mary Lou. University of Miami; Estados Unidos
dc.journal.title
Development
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1242/dev.139220
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://dev.biologists.org/content/144/2/292
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